Pericardium Biological Membrane for Periodontitis

Objective: Heart pericardium membrane allografts are used as a periodontal resorbable membrane which is placed in the sur-gical site to prevent epithelium from growing into the healing site before bone formation and growth of periodontal ligament. Pericardium tissue is shown to be a collagen abundant extracel-lular matrix after decellularization.Materials and Methods: In order to obtain an intact peri-cardium collagen membrane, it should be processed with a technological principle. To avoid any probable disease trans-mission from a donor, the human pericardium is supplied, procured and sterilized under the guidelines of the American Association of Tissue Banks (AATB) . In this research, we pro-cessed the human pericardium with a mild chemical and enzy-matic treatment, and after sterilization and lyophilization, the structure morphology remained, is observed by the Scanning electron microscope.Results: It demonstrates a remarkable parallel fibrous structure and the surface that is suitable for cell reseeding and immigra-tion. Furthermore, histochemical staining by Masson's Tri-chrome (MT) used to evaluate collagen presence and intensity. To investigate the decellularization efficiency, DNA quantifica-tion established to measure the amount of viable cells in peri-cardium membrane allograft. By comparing the native tissue and decellularized membrane, a ۹۱% decrease of DNA content was resulted and showed that the majority of DNA is removed from the tissue and only small amounts are remained.Conclusion: The removal of almost the entire cells which keeps native collagen structural will reduce the possibility of immune responses for applying the decellularized pericardium for the regeneration of all the components of the periodontium, mainly based on guided tissue regeneration (GTR) and guided bone regeneration (GBR).