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مقالات فارسیISIکنفرانسهاژورنالها

Comparative Phytochemical, Nutritional and Antimicrobial Screening of the Seed, Leaf and Root of Vigna Subterranea.

محل انتشار: فصلنامه پیشرفت درتحقیقات بیوشیمی و شیمی، دوره: 5، شماره: 2
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 149

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لینک ثابت به این مقاله:
https://civilica.com/doc/1443335

شناسه ملی سند علمی:

JR_PCBR-5-2_006

تاریخ نمایه سازی: 20 اردیبهشت 1401

چکیده مقاله:

Vigna subterranea, a legume regarded as a ‘super food’ in Nigeria has been grossly understudied and underutilised and this study investigated the constituents of the various parts of this plant for possible utilization. The seed, leaf and root of the plant were analysed to assay the phytochemical constituents using standard gravimetric methods, nutritional and amino acid constituents using both gravimetric and spectroscopic as well as the antimicrobial activities using Agar well diffusion method. The quantitative phytochemical analysis showed high percentage content of alkaloids (۰.۳۴ ±۰.۰۲), flavonoids (۰.۳۷۳ ± ۰.۰۱) and tannins (۰.۳۲۷±۰.۰۱۳) on the leaf followed by the root with the highest saponins content (۰.۴۳۳ ± ۰.۰۲). Steroids were found in moderate quantities on all tested parts. The nutritional analysis showed high protein content on all the parts. The leaf had the highest vitamin C content (۶.۴۵۳mg/۱۰۰g) while vitamin B۳ was found to be highest in the seeds(۲.۱۲۳mg/۱۰۰g) The total essential amino acids (g/۱۰۰g) in seed, leaf and root were ۴۲.۴۴, ۳۹.۸۵, and ۳۰.۸۲ respectively, while total non-essential amino acids (g/۱۰۰g) were ۵۰.۰۵, ۴۶.۸۴, and ۳۸.۲۵ respectively. The antimicrobial analysis showed n-hexane extract of the root having the highest activity against Pseudomonas aeruginosa (۲۴ ± ۱.۴۱ mm zone of inhibition). The Minimum Inhibitory Concentration (MIC) for n-hexane extract was ۱۰۰ mg/L while the Minimum Bacterial Concentration (MBC) was ۲۰۰ mg/L.  indicates a good antimicrobial agent. The leaf and root of Vigna subterranea can no longer be regarded as post -harvest wastes but potential sources of drugs and nutrients.

کلیدواژه ها:

Phytochemical ، Antimicrobial ، Amino acids ، MIC and MBC

نویسندگان

Adaora Ogbuagu

Department of Pure and Industrial Chemistry, Nnamdi Azikiwe University Awka, Anambra state, Nigeria.

Chinwe Maduka

Department of Pure and Industrial Chemistry, Nnamdi Azikiwe University Awka, Anambra state, Nigeria.

Isaac Okerulu

Department of Pure and Industrial Chemistry, Nnamdi Azikiwe University Awka, Anambra state, Nigeria.

Chukwuebuka Onyema

Department of Pure and Industrial Chemistry, Nnamdi Azikiwe University Awka, Anambra state, Nigeria.

Chidi Onyeizugbe

Medical Microbiology unit, Department of Medical Laboratory Science, Iyienu Hospital Ogidi, Anambra state, Nigeria.

Uzoamaka Emezie

Medical Microbiology unit, Department of Medical Laboratory Science, Iyienu Hospital Ogidi, Anambra state, Nigeria.

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