Three-phase Hollow Fiber Liquid-phase Micro Extraction Method for Sertraline Extraction from Biological sample Prior to HPLC-UV Analysis

In This study three-phase hollow fibers liquid-phase micro extraction (LPME) method for preconcentration of sertraline from biological sample was used. Sertraline belongs to the anti depression serotoin reuptake inhibitor drug family which prescribe in a variety of disorder. The extracted analyte with LPME was detected with high performance liquid chromatography equipped with ultraviolet detector (HPLC-UV). The effect of various parameters which affected extraction efficiency such as type of organic solvent, receiving phase, pH of receiving phase and extraction phase, extraction time and mixing speed were investigated. Under optimum condition of extraction condition the pre-concentration factor was obtained ۱۸۰ for urine samples with an appropriate detection limit of ۰.۱ ng ml-۱. The real sample study is provided satisfactory extraction recovery for urine (۹۳%) samples.