Molecular Investigation of Outer Membrane Channel Genes Among Multidrug Resistance Clinical Pseudomonas Aeruginosa Isolates

سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 118

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شناسه ملی سند علمی:

JR_RBMB-11-1_012

تاریخ نمایه سازی: 16 خرداد 1401

چکیده مقاله:

Background: Multidrug resistance Pseudomonas aeruginosa (MDRPA) is most important issue in healthcare setting. It can secrete many virulence effector proteins via its secretion system type (T۱SS-T۶SS). They are using them as conductor for delivering the effector proteins outside to begins harmful effect on host cell increasing pathogenicity, competition against other microorganism and nutrient acquisition.  Methods: The study include investigation of ۵۰ isolates of MDRPA for transport secretion system and resistance for antibiotics. Molecular diagnosis using P. aeruginosa specific primer pairs, investigation of AprF, HasF, XcpQ, HxcQ, PscC, CdrB, CupB۳, and Hcp using specific primer pairs by PCR were also performed. Results: The results revealed high resistance to beta lactam antibiotics (۷۸% for ceftazidime, ۷۸% for cefepime and ۴۶% for piperacillin) can indicate possessing of isolates for beta lactamases and this confirmed by dropping resistance to piperacillin to ۱۶% when combined with tazobactam. Also, the results shown the ability of MDRPA for pyocyanin biosynthesis using the system of genes. Conclusions: The current study conclude that all isolates of P. aeruginosa were highly virulent due to their possessing of all transport secretion system to deliver different effector proteins with possible harmful effects of these proteins.

نویسندگان

Maytham Hassan Jasim Al-Thabhawee

Biology Department, College of Science, University of Babylon, Iraq.

Hussein Oleiwi Muttaleb Al-Dahmoshi

Biology Department, College of Science, University of Babylon, Iraq.

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  • Al-Dahmoshi HO, Al-Khafaji NS, Jeyad AA, Shareef HK, Al-Jebori RF. ...
  • Green ER, Mecsas J. Bacterial secretion systems: an overview. Microbiol ...
  • Chatterjee M, Anju CP, Biswas L, Anil Kumar V, Gopi ...
  • Costa TR, Felisberto-Rodrigues C, Meir A, Prevost MS, Redzej A, ...
  • Maresso AW. Bacterial Secretion Systems, in Bacterial Virulence- A Conceptual ...
  • Liu H, de Souza FZR, Liu L, Chen BS. The ...
  • Létoffé S, Nato F, Goldberg ME, Wandersman C. Interactions of ...
  • Duong F, Bonnet E, Géli V, Lazdunski A, Murgier M, ...
  • Sapriel G, Wandersman C, Delepelaire P. The SecB chaperone is ...
  • Thomas S, Holland IB, Schmitt L. The Type ۱ secretion ...
  • Filloux A. The underlying mechanisms of type II protein secretion. ...
  • Korotkov KV, Gonen T, Hol WG. Secretins: dynamic channels for ...
  • Folders J, Algra J, Roelofs MS, Van Loon LC, Tommassen ...
  • Ball G, Durand E, Lazdunski A, Filloux A. A novel ...
  • Dortet L, Lombardi C, Cretin F, Dessen A, Filloux A. ...
  • Filloux A. Protein secretion systems in Pseudomonas aeruginosa: an essay ...
  • He J, Baldini RL, Déziel E, Saucier M, Zhang Q, ...
  • Hospenthal MK, Costa TRD, Waksman G. A comprehensive guide to ...
  • Christie PJ. The mosaic type IV secretion systems. EcoSal Plus. ...
  • Borlee BR, Goldman AD, Murakami K, Samudrala R, Wozniak DJ, ...
  • Ruer S, Ball G, Filloux A, De Bentzmann S. The ...
  • Silverman JM, Agnello DM, Zheng H, Andrews BT, Li M, ...
  • Ma J, Sun M, Pan Z, Song W, Lu C, ...
  • Varshan R, Prakasam G. Detection of blaVIM gene encoding Metallo ...
  • Al-Byti AM, Chakmakchy SA, Waheeb AA, Alazzawy MA. Multidrug-Resistant Pseudomonas ...
  • Varshitha A, Gopinath P. Detection of bla TEM-۱ gene for ...
  • Abbas MK, Kadhum DA, Shabeeb AK, Mohammed SA. Combination effect ...
  • Mahaseth SN, Chaurasia L, Jha B, Sanjana RK. Prevalence and ...
  • Jyothi P, Shahapur PR, Metri BC. Comparison of various phenotypic ...
  • Saleh MM, Sadeq RA, Latif HKA, Abbas HA, Askoura M. ...
  • Sreeja MK, Gowrishankar NL, Adisha S, Divya KC. Antibiotic resistance-reasons ...
  • Performance standards for antimicrobial susceptibility testing. ۲۹th ed. Clinical and ...
  • Spilker T, Coenye T, Vandamme P, LiPuma JJ. PCR-based assay ...
  • Ozdemir K, Dizbay M, Uğraş Dikmen AS. Incidence and risk ...
  • Li Y, Ren L, Zou J. Risk factors and prevention ...
  • Trinh TD, Zasowski EJ, Claeys KC, Lagnf AM, Kidambi S, ...
  • Karami P, Mohajeri P, Yousefi Mashouf RY, Karami M, Yaghoobi ...
  • Mulcahy LR, Burns JL, Lory S, Lewis K. Emergence of ...
  • Breidenstein EB, de la Fuente-Núñez C, Hancock RE. Pseudomonas aeruginosa: ...
  • Tannous E, Lipman S, Tonna A, Hector E, Hussein Z, ...
  • Winstanley C, Fothergill JL. The role of quorum sensing in ...
  • Hall S, McDermott C, Anoopkumar-Dukie S, McFarland AJ, Forbes A, ...
  • Sabharwal N, Dhall S, Chhibber S, Harjai K. Molecular detection ...
  • Banihashemi K, Sobouti B, Mehregan I, Bakhtiari R, Amirmozafari N. ...
  • Kaviani R, Pouladi I, Niakan M, Mirnejad R. Molecular detection ...
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