The effect of hyperbaric oxygen therapy in the inflammatory response in a mouse model of endometriosis: An experimental study
محل انتشار: مجله طب تولید مثل ایران، دوره: 20، شماره: 5
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 237
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شناسه ملی سند علمی:
JR_IJRM-20-5_001
تاریخ نمایه سازی: 17 خرداد 1401
چکیده مقاله:
Background: Endometriosis pathogenesis is related to the inflammation shown by the secretion of pro-inflammatory mediators. This hypoxia condition can stimulate this condition.
Objective: To investigate the effect of hyperbaric oxygen therapy (HBOT) on the inflammation reaction of endometriosis-induced mice.
Materials and Methods: The animals were designated into ۳ groups: I) the pre-test group, II) the post-test group receiving the HBOT, and III) the post-test group without HBOT. All groups were subjected to induction of endometriosis by xenotransplantation for ۱۵ days. HBOT was given ۳۰ min ۳ times a day for ۱۰ days. The evaluation of the HBOT effect was conducted by examining the endometrial tissue. The inflammation level was evaluated using the Klopfleisch semiquantitative scoring system (index remmele scale), whilst the expression of nuclear factor kappa (NFκB) beta was measured by immunohistochemical staining.
Results: The results showed that group I demonstrated the highest level of inflammation degree (۹.۴۱ ± ۱.۹۹) compared to the post-test groups (group II: ۱.۶۰ ± ۰.۵۳; group III: ۲.۴۲ ± ۰.۵۳). The HBOT-groups was found to have the lowest inflammation level compared to the non-HBOT group (p = ۰.۰۲۰). The results demonstrated that HBOT lowered the peritoneal inflammation degree caused by the endometrial lesion in mice. NFκB expression on the post-test groups was significantly decreased, compared to the pre-test group (p ≤ ۰.۰۰۱), with a strong correlation between the NFκB expression and the peritoneal inflammation level (p ≤ ۰.۰۰۱, r = ۰.۶۷۰).
Conclusion: HBOT significantly reduced the inflammation level on the endometrial lesion in mice, involving the NFκB pathway.
کلیدواژه ها:
نویسندگان
Dedy Syahrizal
Department of Biochemistry, Medical Faculty, Universitas Syiah Kuala, Banda Aceh, Indonesia.
Cut Mustika
Department of Public Health, Medical Faculty, Universitas Syiah Kuala, Banda Aceh, Indonesia.
Nanda Ayu Puspita
Department of Biochemistry, Medical Faculty, Universitas Syiah Kuala, Banda Aceh, Indonesia.
Mohammad Guritno Suryokusumo
Medical Hyperbaric Study Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Hendy Hendarto
Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
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