Mapping QTLs associated with chloride accumulation in leaves of oriental tobacco (Nicotiana tabacum L.) using F۲:۳ population of Basma Seres ۳۱ × SPT ۴۰۶ cross

سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 207

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شناسه ملی سند علمی:

JR_JPMB-7-2_002

تاریخ نمایه سازی: 17 خرداد 1401

چکیده مقاله:

Chloride is considered as the most important micronutrient in tobacco production. But excessive amounts of chloride accumulation in leaves of tobacco have many adverse effects on the tobacco quality, such as burning capacity. Identification of quantitative trait loci (QTL) involved in chloride accumulation would be beneficial for the improvement of tobacco quality. The objective of this study was to identify genomic regions associated with chloride accumulation by using a mapping population consists of ۲۲۵ F۲:۳ families derived from hybridization between ‘Basma Seres ۳۱’ and ‘SPT ۴۰۶’ lines. Linkage map was constructed with ۲۳ microsatellite (SSR) and ۲۹ inter simple sequence repeat (ISSR) polymorphic markers which covered ۵۷۰.۸ cM of the tobacco genome. Thirty-four of these polymorphic markers were mapped to ۷ linkage groups. Distance between two adjacent markers was ۱۷.۳ cM. Composite interval mapping (CIM) was used to identify QTLs controlling chloride accumulation. One QTL for chloride accumulation was identified on linkage group ۳. The percentage of phenotypic variance (R۲) explained by this QTL was ۱۲.۷%. A significant association was not found between ISSR markers and chloride accumulation. The outcome of present effort can be a basis for marker aided selection (MAS) in tobacco breeding programs.

نویسندگان

Faramarz Hoshyardel

Department of Plant Production and Genetics, Faculty of Agriculture, Urmia University, Urmia, Iran

Reza Darvishzadeh

Department of Plant Production and Genetics, Faculty of Agriculture, Urmia University, Urmia, Iran

Hamid Hatami Maleki

Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, Iran

Marjan Jannatdoust

Department of Plant Production and Genetics, Faculty of Agriculture, Urmia University, Urmia, Iran

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