Hsa-miR-۲۴ is a novel and critical regulatory biomarker of LBL by regulation of cell growth signaling pathway: integrated microarray and bioinformatics analyses

Lymphoblastic leukemias lymphomas (B-All / LBL) account for ۲% of lymphoid neoplasms of precursor TCellsor lymphoblasts. The incidence appears to be rising in both children and adults. B-Cells acutelymphoproliferative disease manifest as pure leukemia (B-All) in ۸۰% of cases, isolated extramedullarypresentations frequently have marrow involvement at diagnosis that may be morphologically biomarker mayrequire detection by high-resolution flow cytometry. In recent decades, miRNAs and lncRNAs have beenstudied and are considered impactful biomarkers in cancer. Therefore, in this bioinformatic approach, thegoal was to spot and determine a biological network of genes, miRNAs, and lncRNAs, which have a notableinfluence on progression LBL. Microarray data analysis of the LBL gene expression profile was performedby GEO۲R online software. GSE۲۹۹۸۶ was analyzed in this study. Using miRWalk and miRdSNP,microRNA – mRNA interaction analysis was performed. The common microRNAs were selected by venny۲.۱ online software. Pathway enrichment and mRNA interaction analyses were performed by DAVID. Singlenucleotide polymorphism analysis was performed by miRNASNP v۳. Based on microarray analysis byGEO۲R, PDGFRB has a significant dysregulation in the LBL samples compared to control (adj. p. value <۰.۰۰۰۱). DAVID database revealed that PDGFRB and the related mRNAs, EBF۱, LPAR۱, WNT۶, and FN۱,are the essential genes in the cell energy and growth signaling pathway. Also, hsa-miR-۲۴ is the commonlyinteracted microRNAs with all of mentioned five genes, and its binding affinity is correlated to the rs۲۴۶۳۸۷.In conclusion, hsa-miR-۲۴ regulates the cell growth pathway and LBL development by suppressing theexpression of mentioned genes, especially in the rs۲۴۶۳۷۸ region of the PDGFRB gene.