The Effect of Fasting on Oxidative Stress in the Vital Organs of New Zealand White Rabbit

Novi Silvia Hardiany; Adrian Prasetya Karman; Aisha Safa Putri Calista; Budi Gittanaya Anindyanari; Daniell Edward Rahardjo; Puti Retasya Novira; Rania Rifdah Taufiq; Shofiyya Imtiyaz; Radiana Dhewayani Antarianto

The Effect of Fasting on Oxidative Stress in the Vital Organs of New Zealand White Rabbit

محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 11، شماره: 2

سال انتشار: 1401

نوع سند: مقاله ژورنالی

زبان: انگلیسی

مشاهده: 109

فایل این مقاله در 10 صفحه با فرمت PDF قابل دریافت می باشد

دریافت فایل کامل مقاله

صدور گواهی نمایه سازی
من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

https://civilica.com/doc/1495037

شناسه ملی سند علمی:

JR_RBMB-11-2_001

تاریخ نمایه سازی: 21 مرداد 1401

چکیده مقاله:

Background: Oxidative stress is defined as the condition in which balance between the synthesis and detoxification of reactive oxygen species in cells is disrupted. This research explored the effects of intermittent and prolonged fasting on malondialdehyde (MDA), carbonyl, reduced glutathione (GSH), and specific activity of catalase as biomarkers for oxidative stress in hearts, brains, and kidneys of New Zealand White (NZW) rabbits. Methods: Fifteen NZW rabbits were divided into control, intermittent fasting (IF), and prolonged fasting (PF) groups. The controls were fed ad lib. IF and PF groups were fasted for ۱۶ and ۴۰ hours, respectively, followed by eight hours of non-fasting, for six days and were sacrificed on the ۷th day. One hundred mg of heart, brain, and kidney tissues were homogenized in ۱ ml of phosphate-buffered saline. MDA, carbonyl, GSH, and catalase were analyzed by spectrophotometry. Data were analyzed using One-way ANOVA and post hoc test. Results: In heart, MDA was significantly greater in the control than in the IF and PF groups. In brain, GSH was greater in the IF than in the PF and control groups. Also, in brain, catalase specific activity was significantly greater in the control than in the IF and PF groups. In kidney, catalase specific activity was significantly less in the PF than in the control group. Conclusions: The effect of fasting on oxidative stress in various organs showed various responses, however fasting reduced oxidative stress based on MDA and GSH levels in the heart and brain, respectively.

کلیدواژه ها:

Fasting ، Oxidative stress ، Vital organs ، Rabbit

نویسندگان

Novi Silvia Hardiany

Department of Biochemistry & Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia & Center of Hypoxia & Oxidative Stress Studies, Department of Biochemistry & Molecular. Biology, Faculty of Medicine Universitas Indonesia, Jaka

Adrian Prasetya Karman