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مقالات فارسیISIکنفرانسهاژورنالها

Kinetic Changes of Ptdins (۳,۴,۵) P۳ within Fast and Slow Turnover Rates of Focal Adhesion

محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 11، شماره: 2
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 165

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https://civilica.com/doc/1495045

شناسه ملی سند علمی:

JR_RBMB-11-2_009

تاریخ نمایه سازی: 21 مرداد 1401

چکیده مقاله:

Background: The assembly and disassembly of the focal adhesions (FA) components occurs throughout life cycle of adhesion, with conservation of balance between removal and recruitment rate during temporal stages. Previous studies have demonstrated that phosphotidyilinositols play a role in regulating FA turnover. However, a little attention has been given to quantify the dynamics changes of Phosphatidylinositol ۳,۴,۵-trisphosphate (PtdIns (۳,۴,۵) P۳) within and during fast and slow turnover rates of FA. Methods: MDA-MB-۲۳۱ breast cancer cell line was used as a model in this study due to high metastatic and motile. These cells were co-transfected with GFP- paxillin/vinculin, as FA marker, and the GFP/mCherry-Btk-PH, as a biosensor to visualize PtdIns (۳,۴,۵) P۳. Confocal time-lapse images were used to monitor changes or differences in the local generation of PtdIns (۳,۴,۵) P۳ within and during assembly and disassembly of FA. Following transfection, immunostaining was used to examine the spatial colocalization between FA and PtdIns (۳,۴,۵) P۳. Results: Our data demonstrated that PtdIns (۳,۴,۵) P۳ co-localized with FAs and increase during assembly and decline during disassembly of FA which exhibits slow turnover rates and was in a constant level during assembly and disassembly of FA that displays fast turnover rates.  Conclusions: Our result suggested that the dynamic changes of PtdIns (۳,۴,۵) P۳, it may depend on components undergo turnover, such that early, nascent FA displays fast turnover rates and mature FA exhibits slow turnover rates. Thus, the local enrichment of PtdIns (۳,۴,۵) P۳ enhances FA assembly and disassembly activation.

کلیدواژه ها:

Cancer cell migration ، Focal adhesion turnover ، MDA-MB-۲۳۱ cell line ، PtdIns (۳ ، ۴ ، ۵) P۳.

نویسندگان

Dhurgham Al-Fahad

Department of Pharmaceutical Sciences, College of Pharmacy University of Thi-Qar, Thi-Qar ۶۴۰۰۱, Iraq.

Firas Alyaseen

Department of Pharmaceutical Sciences, College of Pharmacy University of Thi-Qar, Thi-Qar ۶۴۰۰۱, Iraq.

Ahmed Al-Amery

epartment of Physiology, College of Medicine, University of Thi-Qar, Iraq.

Gagandeep Singh

Viral Research and Diagnostic Laboratory, Department of Microbiology, Osmanian Medical College, Hyderabad, Telangana, India & Kusuma School of Biological Sciences, Indian Institute of Technology Delhi, New Delhi, India.

Mote Srinath

Viral Research and Diagnostic Laboratory, Department of Microbiology, Osmanian Medical College, Hyderabad, Telangana, India.

Yahya Abbas

Department of Biology, College of Science, University of Thi-Qar, Thi-Qar, ۶۴۰۰۱ Iraq.

Hafiz Muzzammel Rehman

School of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan.

Yahya Abbas

Department of Biology, College of Science, University of Thi-Qar, Thi-Qar, ۶۴۰۰۱ Iraq.

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