Protective Effect of Various Extracts of Allium hirtifolium and Satureja khuzestanica Plants on AAPH-Induced Oxidative Hemolysis

Background: Allium hirtifolium and Satureja khuzestanica are the Iranian endemic plants and propercandidates for antioxidant studies. This study investigated the antioxidant, anti-hemolytic properties, andphytochemical composition in different extracts of A. hirtifolium and S. khuzestanica.Methods: Hydroalcoholic, methanolic, and n-hexane extracts of A. hirtifolium and S. khuzestanicaplants were prepared using soaking and ultrasonic methods. Different plant extracts were evaluatedfor the presence of secondary metabolites using standard methods based on colorimetric analysis. Theantioxidant properties of the compounds were measured by the ferric reducing ability of power (FRAP)and ۲,۲-diphenyl-۱-picrylhydrazyl (DPPH) methods. Finally, the anti-hemolytic effects of the extractswere investigated using the ۲,۲’-Azobis(۲-amidinopropane) dihydrochloride (AAPH) methods.Results: Phytochemical analysis indicated the presence of flavonoids, phenolic acids, glycosides, steroids,and terpenoids in S. khuzestanica, as well as amino acid compounds, flavonoids, saponins, glycosides,and phenols in A. hirtifolium. FRAP assay showed that the methanolic extract of S. khuzestanica and then-hexane extract of A. hirtifolium have the highest total antioxidant activity. The results of the DPPH assayalso demonstrated that the minimum IC۵۰ was related to the hydroalcoholic extract of S. khuzestanica(۱۸.۵۸ μg/mL) and the n-hexane extract of A. hirtifolium (۸۷.۹۵ μg/mL). In general, the extracts of bothplants could reduce the percentage of AAPH-induced hemolysis while being significant only in someconcentrations (P < ۰.۰۰۱).Conclusion: Overall, A. hirtifolium and S. khuzestanica can be used as a herbal supplement in the humandiet due to their anti-hemolytic effects.