Investigation of acute pre-workout supplement ingestion with resistance exercise on the responses of Inflammatory muscle factors in athlete men

Some activities, such as intense resistance exercise, can increase the enzymes of muscle inflammation in the blood. Some previous study has shown some pre-workout supplements may reduce inflammation and pain due to their ingredients. The purpose of this study was to examine the influence of ingestion of the pre-workout C۴ supplement on muscle inflammation responses by analyzing creatine kinase and lactate dehydrogenase in athletes’ men.We have chosen eight male physical education students (age: ۲۲.۹±۱.۷ yr, body fat: ۱۲.۷±۱.۹, and BMI: ۲۳.۱±۱.۷ kgm۳) in a randomized, double blind, placebo controlled, and crossover method design. the exercise protocol was including ۷ exercises whole-body movement that performed in ۷۵% ۷RM for ۳ set with ۱۰ repetitions in each set. Participants consumed supplements or placebo ۳۰ minutes before exercise. The supplement was a single scoop (۶.۵ gr) and the placebo was ۶.۵ gr maltodextrin. Blood samples were taken before, immediately post, and ۱hour post-resistance exercise to analyze Creatine Kinase (CPK) and Lactate dehydrogenase (LDH). For analysis of data, the repeated-measures ANOVA and Bonferroni tests were used.The results had shown that there were no significant differences in acute muscle inflammation responses between C۴ consumption and placebo-controlled men. Lactate dehydrogenase levels, immediately after ingestion and ۱ hour after resistance exercise don’t show any differences between placebo and supplement conditions (p>۰.۰۵). However, the rise of Creatine Kinase after exercise was higher in placebo-controlled than supplement conditions (p<۰.۰۵).According to the results of this study, the C۴ supplement has no effective response on reducing lactate dehydrogenase accumulation after resistance exercise but can affect the decrease of creatine kinase levels, So this supplement improves the recovery conditions of the body after exercise.