Is it possible to restore the reduced coenzyme Q۱۰ production of a varied strain of Gluconobacter?
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 136
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شناسه ملی سند علمی:
JR_IJGPB-10-1_003
تاریخ نمایه سازی: 2 مهر 1401
چکیده مقاله:
Different strains of a bacterial culture can undergo phenotypic variation upon continuous passages. These changes often cause a reduction or loss of bacterial metabolic potential and ultimately lead to a decrease in production yield. The aim of this study was to address this question; is it possible to restore the reduced coenzyme Q۱۰ production of a varied strain of Gluconobacter to a higher level? The main strain studied in this study was Gluconobacter japonicus FM۱۰, from which the FM۲۰ strain was obtained. FM۲۰ strain was a varied strain whose ability on coenzyme Q۱۰ production was reduced during some continuous passages. FM۳۰ strain was obtained from FM۲۰ strain by restricting the available oxygen. The amount of coenzyme Q۱۰ produced by all three strains was measured. The activities of glycerol dehydrogenase and sorbitol dehydrogenase were also measured. The results showed that coenzyme Q۱۰ production in FM۳۰ strain that had been exposed to restricted oxygen was several times higher (۶.۳ mg/L) than the varied FM۲۰ strain (۰.۸ mg/L), and even the original FM۱۰ strain (۲.۷ mg/L). The investigation of the enzymes activities showed that glycerol dehydrogenase and sorbitol dehydrogenase activities of FM۳۰ strain were higher than those of the others (۰.۶۶ and ۰.۵۲ U mg-۱, respectively). It can be concluded that by using some strategies, the metabolic potential of some varied strains can be restored. For strictly aerobic bacteria, Gluconobacter, the oxygen restriction may be an effective strategy for the restoration of coenzyme Q۱۰ production ability.
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نویسندگان
فوزیه مقدمی
Department of Biology, Payame Noor University, Tehran, Iran.
رامین حسینی
Department of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran.
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