Background and Aim : Bacterial infections and their transmission are a significant threat tohuman health, and controlling the harmful effects of microorganisms in recent years has becomeunavoidable. Currently, the treatment of these infections is mainly limited to the use of antibiotics,which has inevitably led to the rapid emergence of antibiotic-resistant bacterial species in the pastfew decades. Therefore, developing a new strategy to inactivate bacteria is necessary and of greatimportance. One of the materials that has attracted the attention of many scientists in recent yearsdue to its similarity to graphene as a non-metallic material is
nanosheet carbon nitride (g-C۳N۴Nanosheet). The purpose of this study is to synthesize and investigate the antibacterial propertiesg-C۳N۴ Nanosheet.Methods : In order to synthesize carbon nitride nanosheets, the melamine precursor was subjectedto heat treatment in an electric furnace. Then, XRD, FE-SEM, BET, FTIR and UV-VISIBLEcharacterization tests were used to identify and check the properties of nanomaterials. Finally, theantibacterial properties of g-C۳N۴ Nanosheet against Gram-positive and Gram-negative bacteriawere evaluated using the Colony Count method, and MIC and MBC method.Results : The analyzes performed while confirming the size and structure of the synthesized nanomaterial showed the separation of the carbon nitride plates. Also, the results of the Colony Counttest showed that the colony density of both bacterial strains decreased after treatment withnanomaterials. In addition, the minimum bacterial growth inhibition concentration (MIC) andminimum bactericidal concentration (MBC) of carbon nitride nanosheets for each of bacteria wasequal to ۲۵ μg/mL.Conclusion : One of the reasons that g-C۳N۴ Nanosheet can destroy bacteria is that the sharpedges of the nanomaterial lead to the rupture of the bacterial membrane and its death. The carbonnitride nanosheets synthesized in this study can be mentioned as a nano material with uniquefeatures such as high biocompatibility, low cost and simple synthesis, and high speed of theprocess.