Type III Secretion System (Exoenzymes) as a Virulence Determinant in Pseudomonas aeruginosa Isolated from Burn Patients in Mansoura University Hospitals, Egypt
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 154
فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJMM-16-6_003
تاریخ نمایه سازی: 7 آبان 1401
چکیده مقاله:
Background and Aim: Pseudomonas aeruginosa (P. aeruginosa) is an important causative organism of burn infection. Several virulence factors are implicated in P. aeruginosa colonization and invasion, making P. aeruginosa infection's outcome worse. Type III secretion system (T۳SS) effector proteins are among these virulence factors. The present study evaluated the frequency of genes encoding T۳SS effectors as a virulence determinant in P. aeruginosa isolates from burn patients.
Materials and Methods: Wound swabs were collected from burn patients admitted to the Plastic and Reconstructive Surgery Center, Mansoura University, Egypt, and identified by different microbiological testing methods. The modified Kirby Bauer's disc diffusion method was used to test the antibiotic susceptibility of P. aeruginosa isolates against different antibiotics. Prevalence and the presence of exo genes that encode type T۳SS proteins (exoS, exoT, exoU, and exoY) in P. aeruginosa isolates were evaluated by the multiplex PCR. Chi-square and Fisher's test were used for statistical analysis.
Results: A total of ۴۵ P. aeruginosa isolates were identified from ۱۰۱ burn patients, including ۲۷ males and ۱۸ females, with a mean age of ۱۵.۷۸±۲.۶۵ years old. P. aeruginosa isolates were mostly susceptible to piperacillin/tazobactam and imipenem (۷۳.۳۳ and ۶۲.۲۲%), respectively; while the lowest susceptibility rates were in ceftazidime (۴.۴۴%), Tobramycin (۴.۴۴%), and ceftriaxone (۶.۶۷%). The exoY and exoT genes were detected in ۱۰۰% of the P. aeruginosa isolates, while ۶۲.۲۲% and ۴۲.۲۲% of clinical isolates harbored exoS and exoU genes, respectively.
Conclusion: This study established a correlation between T۳SS proteins, particularly exoS and exoU genes and antimicrobial resistance in P. aeruginosa isolates from burn infection.
کلیدواژه ها:
Type III secretion system ، Pseudomonas aeruginosa ، Antimicrobial resistance ، Burn infection ، سیستم ترشحی نوع III ، سودوموناس آئروژینوزا ، مقاومت ضد میکروبی ، عفونت سوختگی
نویسندگان
Rasha Mokhtar Elnagar
Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Mohammed Elshaer
Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Omar Osama Shouman
Department of Plastic and Reconstructive Surgery, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Samah Sabry El-Kazzaz
Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
