Research Article: Hepatic CYP۴۵۰ gene expression, hematological and biochemical indices in Caspian roach (Rutilus caspius) induced by Endosulfan

Endosulfan is one of the organochlorine pesticides which has been used worldwide for decades. The purpose of the present study was to investigate the effect of endosulfan on hematological and P۴۵۰ gene expression in Rutilus caspius. Fish were exposed to the ۱۰% and ۲۰% LC۵۰ for ۲۱ days and were sampled on days ۱, ۷, ۱۴ and ۲۱. Results showed that there were significant differences in hematological parameters among the control and treated fish (p<۰.۰۵). The highest amount of Hb, Ht and RBC was observed in the control, while WBC was highest in the ۲۰% endosulfan treated group on day ۲۱. According to the results alkaline phosphatase, aspartate aminotransaminase and alanine amino transaminase levels increased during the experiment. The highest levels were observed with ۲۰% endosulfan on day ۱۴ which were significantly different with those in the control (p<۰.۰۵). In addition, the CYP۴۵۰ gene expression had the same results. We conclude that exposure to the endosulfan (especially ۲۰%) can enhance the innate immune system in Rutilus caspius.