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Elucidation of flavonoids from potent Iranian Scutellaria species against Influenza A (H۱N۱) virus

محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 26، شماره: 1
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 145

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استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1574121

شناسه ملی سند علمی:

JR_IJBMS-26-1_010

تاریخ نمایه سازی: 8 دی 1401

چکیده مقاله:

Objective(s): Influenza A virus (IAV) is a contagious illness. Different species of Scutellaria genus are used as a traditional remedy to reduce influenza symptoms. This study aimed to investigate the anti-influenza capacity of several species of Iranian Scutellaria and identify active compounds of the most potent species for the first time.Materials and Methods: Some Iranian species of Scutellaria were collected from different regions of Iran, including S. pinnatifida with mucida, viridis, and alpina subspecies; S. tournefortii; S. tomentosa; S. persica. They were fractionated to chloroform and methanol. The total phenols and flavonoids of samples were examined by the folin-ciocalteau and aluminum-flavonoid complex methods, respectively. The ۵۰% cytotoxic concentrations (CC۵۰) on MDCK cells and non-cytotoxic concentrations (NCTC) were determined by MTT assay. The percentage of cell protection against IAV and their effect on virus titer were investigated in pre-, post-, and co-penetration treatment groups. Phytochemicals of the most effective species were isolated by various chromatographic methods and identified by different spectroscopic methods.Results: Methanol fraction of S. pinnatifida subsp. viridis demonstrated the highest amounts of flavonoid content and best activity against influenza A virus in all combination treatments, which reduced the virus titer by ۵ logs with no cytotoxicity. Kaempferol-۳-O-glucoside, quercetin-۳-O-glucoside, apigenin-۴′-methoxy-۷-O-glucoside, luteolin, and luteolin-۷-O-glucoside were purified and identified from this species.Conclusion: Scutellaria pinnatifida subsp. viridis can be introduced as a source of flavonoids with acceptable anti-influenza activity. S. tomentosa also showed potent antiviral effects and is a candidate for elucidation in further studies.

کلیدواژه ها:

Antiviral ، Flavonoids ، Folin-ciocalteau reagent ، H۱N۱ virus ، MTT Assay ، Scutellaria

نویسندگان

Mostafa Pirali Hamedani

Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Mahtab Ahmad Kashi

Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Saied Goodarzi

Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Hadiseh Shokouhi

Influenza and Respiratory Viruses Department, Pasteur Institute of Iran, Tehran, Iran

Mehdi Shafiee Ardestani

Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Abbas Hadjiakhoondi

Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Morteza Pirali Hamedani

Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Mohammad Hosein Ghahremani

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Parvaneh Mehrbod

Influenza and Respiratory Viruses Department, Pasteur Institute of Iran, Tehran, Iran

Zahra Tofighi

Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

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