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مقالات فارسیISIکنفرانسهاژورنالها

The Radioprotective Effect of Magnesium Sulfate and Vitamin A on Radiation-induced Micronuclei and the Expression of NOX۴ in Bone Marrow Cells of Mice

محل انتشار: مجله دانشگاه علوم پزشکی کرمان، دوره: 29، شماره: 3
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 138

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لینک ثابت به این مقاله:
https://civilica.com/doc/1582320

شناسه ملی سند علمی:

JR_JKMU-29-3_003

تاریخ نمایه سازی: 19 دی 1401

چکیده مقاله:

Background: Radioprotectors are used to neutralize the effects of free radicals caused by ionization radiation. In this study, the radioprotective effects of magnesium sulfate and vitamin A on bone marrow cells of mice were evaluated by micronucleus assay and changes in the expression of NOX۴ gene.Methods: The mice were randomly divided into ۱۲ groups. The mixture of drugs was injected into mice by intraperitoneal injection ۲ hours before the irradiation. The dose rate was ۵۰ cGy/min at SSD (source to surface distance) ۱۰۰ cm and field size of ۱۰×۱۰cm۲. Twenty four hours after ۲ Gy irradiation by LINAC, the mice were sacrificed by cervical dislocation. Then, several microscopic slides were prepared for each sample to evaluate the number of micronucleus in polychromatic erythrocytes (PCEs). In addition, the expression of NOX۴ was evaluated by Real-time PCR. Data were analyzed through SPSS ۱۹ and the mean of groups was compared to each other using one-way ANOVA.Results: There was a significant difference between mean mnPCEs in the treatment (drugs + radiation) groups compared to the ۲ Gy group (P=۰.۰۱). The expression level of NOX۴ gene was significantly lower in groups receiving the combinations of vitamin A and magnesium sulfate compared to the ۲ Gy group (P =۰.۰۱). The calculated dose reduction factor (DRF) demonstrated DRF=۲.۵۸ for ۲Gy.Conclusion: The results of this study indicated that the combination of vitamin A and magnesium sulfate, possibly with an antioxidant mechanism, removes the deleterious effects of free radicals caused by ionizing radiation on bone marrow cells.

کلیدواژه ها:

Keywords: Micronucleus assay ، magnesium sulfate ، NOX۴ ، Radiation-protective agents ، Vitamin A

نویسندگان

Mohammad Mirdoraghi

Department of Medical Physics and Biomedical Engineering, Tehran University of Medical Sciences, Tehran, Iran

Vahid Changizi

Department of Radiology and Radiotherapy Technology, School of Allied Health Sciences, Tehran University of Medical Sciences, Tehran, Iran

Seyed Abolghasem Haeri

Nuclear Science and Technology Research Institute, Tehran, Iran

Zahra Rajabi

Zoonosis Research Center, Tehran University of Medical Sciences, Tehran, Iran

Peyman Amini

Department of Radiology and Radiotherapy Technology, School of Allied Health Sciences, Tehran University of Medical Sciences, Tehran, Iran

Sakineh Abassi

Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Tehran University of Medical Sciences, Iran

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