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مقالات فارسیISIکنفرانسهاژورنالها

Comparison of Poly(Lactic-Co-Glycolic Acid) and Poly(Lactic-Co-Glycolic Acid)/Hyaluronic Acid Based Scaffolds for Chondrogenesis of Human Adipose-derived Stem Cells by Avocado/Soybean Unsaponifiables

محل انتشار: مجله دانشگاه علوم پزشکی کرمان، دوره: 28، شماره: 3
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 154

فایل این مقاله در 9 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1582619

شناسه ملی سند علمی:

JR_JKMU-28-3_008

تاریخ نمایه سازی: 19 دی 1401

چکیده مقاله:

Background: Corporation of Hyaluronic acid (HA) with PLGA is an effective way to potentially enhance chondrogenesis. The aim of this study was to use HA macroporous biodegradable poly(lactic acid-co-glycolic acid) [PLGA] scaffold to enhance the attachment, proliferation and differentiation of chondrocytes for cartilage tissue engineering and articular cartilage regeneration of human adipose derived stem cells (hADSCs) in the presence of avocado/soybean unsaponifible (ASU).Methods: The PLGA and PLGA/HA scaffolds were prepared and hADSCs were cultured separately on the scaffolds and ۱۴ days after differentiation, chondrogenic genes in each scaffold evaluated using real time PCR and cell viability examined by (۳-(۴,۵-dimethylthiazol-۲-yl)-۲,۵-diphenyltetrazolium bromide (MTT) assay.Results: The viability and proliferation of cells in-group of PLGA significantly decreased in comparison with the control (P=۰.۰۰۲) and PLGA/HA (P=۰.۰۱۳) groups.The expression of (SOX۹), Aggrecan (AGG), and Collagen type II (Col II) genes was significantly higher in the PLGA and PLGA/HA groups compared to the control group (P≥۰.۰۵).The gene expression of SOX۹ (P=۰.۰۰۳) and AGG (P=۰.۰۰۹) was significantly higher in the PLGA/HA groups compared to the PLGA group. The results of real time PCR showed that collagen type X (Col X) gene expression in the PLGA group, was significantly higher than the control and PLGA/HA groups (P=۰.۰۰۰).Conclusion: The corporation of HA with PLGA is an effective way to potentially enhance chondrogenesis and articular cartilage regeneration of hADSCs in the presence of avocado/soybean unsaponifiables (ASU).

کلیدواژه ها:

Hyaluronic Acid ، Poly (Lactic-Co-Glycolic) Acid ، Chondrogenesis ، Human Adipose-derived Stem Cell ، Scaffold

نویسندگان

Batool Hashemibeni

Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran

Zynolabedin Sharifian

Department of Anatomy, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran

Ali Valiani

Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran

Hengameh Dortaj

Department of Tissue Engineering and Applied Cell Science, Shiraz University of Applied Medical Science and Technologies, Shiraz, Iran

Mohammad Zamani Rarani

Department of Anatomy, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran

Mohammad Mardani

Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran

Majid Pourentezari

Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

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