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مقالات فارسیISIکنفرانسهاژورنالها

Comparison of Fibrin and PLGA/fibrin Scaffolds for Chondrogenesis of Human Adipose Derived Stem Cells by Icariin

محل انتشار: مجله دانشگاه علوم پزشکی کرمان، دوره: 27، شماره: 1
سال انتشار: 1399
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 105

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لینک ثابت به این مقاله:
https://civilica.com/doc/1582701

شناسه ملی سند علمی:

JR_JKMU-27-1_002

تاریخ نمایه سازی: 19 دی 1401

چکیده مقاله:

Background: Recently, cartilage tissue engineering is the best candidate for regeneration of cartilage defects. We evaluated the potential of fibrin and PLGA/fibrin scaffolds in providing a suitable environment for growth and chondrogenic differentiation of human adipose derived stem cells (hADSCs) in the presence of icariin. Method: The Three-dimensional (۳-D) PLGA scaffold was prepared using the solvent casting/salt leaching technique and the hybrid scaffold was fabricated by fibrin. hADSCs were isolated from human adipose tissue. ۳-D PLGA/fibrin scaffolds were seeded with cultured hADSCs and analyzed ۱۴ days later, Monolayer culture was used for the control group. The viabilities of cells in different groups were assessed by MTT. The expression of chondrogenic related genes, hypertrophic marker and Fibrotic marker were quantified by RT-PCR. Results:MTT results show that viability in the control group was significantly higher than those in the Fibrin and PLGA/Fibrin groups. Also viability in the PLGA/Fibrin group affected by icariin was higher than that in Fibrin group. The results of the real-time PCR showed that SOX۹, Agg, Coll ۲, and Coll ۱ gene expression in the fibrin and PLGA/fibrin groups were significantly higher than those in the control group. Coll ۱۰ gene expression in the fibrin group was higher in comparison to the control group but not significantly. type SOX۹, Coll ۲ and Coll ۱ gene expression in the fibrin group was significantly lower compared to the PLGA/fibrin group. Conclusions: The study reveals that the corporation of PLGA with fibrin is an effective way to potentially enhance articular cartilage regeneration of hADSCs in the presence of icariin.

کلیدواژه ها:

Icariin ، Chondrogenesis ، Human Adipose Derived Stem Cell (hADSC) ، Fibrin ، Poly (lactic-co-glycolic) Acid/Fibrin ، Scaffold

نویسندگان

Batool Hashemibeni

Professor, Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran

Mohammad Mardani

Professor, Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran

Maryam Bahrami

Ph.D. Candidate, Anatomy Department, Medical Faculty, Shaheed Beheshti University of Medical Sciences, Tehran, Iran

Ali Valiani

Associate Professor, Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran

Mohsen Setayesh Mehr

Assistant Professor, Nanotechnology and Tissue Engineering Group, Department of Advanced Medical Technology, Isfahan University of Medical Sciences, Isfahan, Iran

Majid Pourentezari

Assistant Professor. Department of Anatomical Sciences and Molecular Biology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

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