Determination of Erythromycin in Milk Samples by Nano Magnetite Hexadecylsilane by LC-Tandem Mass Spectrometry
سال انتشار: 1399
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 122
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شناسه ملی سند علمی:
JR_ANALCH-7-3_010
تاریخ نمایه سازی: 16 بهمن 1401
چکیده مقاله:
Erythromycin is one of the typical macrolides, which is isolated by the Saccharopolyspora erythraea. It has been listed in WHO essential medicine for improvement of the efficiency of health system. In this study, magnetic iron nanoparticles were coated with cetyl as a non-polar functional group and characterized by various techniques such as Infrared Spectroscopy, thermal gravimetric analysis, scanning electron microscopy and vibrating sample magnetometer. The as-prepared nanoparticles were used to extract erythromycin from the milk samples. Separation was performed on a pentaflurophenyl column (۱۵۰*۲mm,۳μm) using a mobile phase consisting ۷۰% acetonitrile and ۳۰% ammonium acetate (۱۰mM, pH۳.۵) with a high performance liquid chromatography system coupled with tandem mass spectrometry. The separation was fast and completed in less than ۵ minutes, under the optimized condition. Stable isotope of erythromycin was used as internal standard in the sample preparation and calibration curve. It was found that relative recovery of the method was ۹۲.۶%. The proposed method was convenient and quick preparation method was achieved using external magnetic field without centrifugation and filtration. The detection limit and coefficient of determination were ۲.۴ μg/L and R۲ = ۰.۹۹۸۳, respectively. The intra- and inter-day precisions of the proposed method in different levels of spiked sample were in the range of ۵.۶-۸.۵% and ۸.۴-۱۲.۵%, respectively.
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نویسندگان
Abdollah Tavassoli
Analytical of Chemistry, University of Mazandaran, Babolsar, Iran
Seyed Naser Azizi
Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, ‎Babolsar, Iran
Abdolraouf Samadi-Maybodi
Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran
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