Identification of Potential Antioxidant and Hypoglycemic Compounds in Aqueous-Methanol Fraction of Methanolic Extract of Ocimum canum Leaves

Chimaobi Ononamadu; Godwin O. Ihegboro; Tajudeen A. Owolarafe; Kailani Salawu; Muhjib Fadilu; Obiajulu C. Ezeigwe; Moses L. Oshobu; Francis C. Nwachukwu

Identification of Potential Antioxidant and Hypoglycemic Compounds in Aqueous-Methanol Fraction of Methanolic Extract of Ocimum canum Leaves

محل انتشار: مجله تحقیقات شیمی تجزیه و تجزیه زیستی، دوره: 6، شماره: 2

سال انتشار: 1398

نوع سند: مقاله ژورنالی

زبان: انگلیسی

مشاهده: 144

فایل این مقاله در 9 صفحه با فرمت PDF قابل دریافت می باشد

دریافت فایل کامل مقاله

صدور گواهی نمایه سازی
من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

https://civilica.com/doc/1598264

شناسه ملی سند علمی:

JR_ANALCH-6-2_015

تاریخ نمایه سازی: 16 بهمن 1401

چکیده مقاله:

Ocimum canum belongs to a genus of aromatic annual and perennial herbs and shrubs in the family of Lamiaceae. It is native to Africa and cultivated commonly for its culinary and medicinal value. In a previous study, we reported the antioxidant and hypoglycemic activity of the crude methanol extract and the solvent fractions of Ocimum canum leaves.This present study was aimed to partially isolate and identify some bioactive compounds in the aqueous-methanol solvent fraction of methanol extract of Ocimum canum leaves. The sample was fractionated via silica gel-column and solvent system to yield many fractions which was pooled using analytical TLC. The pooled fractions were screened for antioxidant activity (DPPH scavenging assay and Ferric Reducing Power) and ability to reduce fasting blood glucose (FBG). The most active sub fraction was analysed with LC-MS and GC-MS to identify the active compounds. A total of ۱۹۷ fractions were collected and pooled to seven (۷) subfractions. Subfraction OC۴ showed the best activity with IC۵۰s ۴۸.۶±۶.۶µg/ml and ۱۵.۲±۳.۹µg/ml for DPPH scavenging and Ferric Reducing Power assays respectively, a ۳۷% reduction in FBG and a concomitant increase in insulin level in treated diabetic rats. The LC/GC-MS analysis revealed tentatively the following compounds: Diosmetin, Dihydroquercetin, Digalloyl glucose isomer, Ananaflavoside isomer, Kaempferol glucoside embinin isomers, eugenol, hexadecanoic acid, ۹,۱۲ octadecanoic acid, and β-sitosterol. The study corroborates the previously reported antioxidant activity and hypoglycemic potential of the leaves of Ocimum canum and further more identified bioactive compounds that can be isolated and further characterised.

کلیدواژه ها:

Ocimum canum ، Hypoglycemic ، Antioxidant ، LCMS ، GCMS

نویسندگان

Chimaobi Ononamadu