Isolation and Characterization of Lytic Bacteriophages from Wastewater against Listeria monocytogenes
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 130
فایل این مقاله در 9 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_JIML-9-1_003
تاریخ نمایه سازی: 3 اسفند 1401
چکیده مقاله:
Background and Aims: In the current study, two bacteriophages were isolated from the wastewater sources and characterized by lytic activity against the Listeria monocytogenes (L. monocytogenes) bacterium.
Materials and Methods: ۵۰ wastewater samples were collected from the disposal sites. The phages were isolated from the treated samples using the double agar overlay method with L. monocytogenes as the host bacterium. Plaque morphology, temperature, pH, titration of phage lysate, host range, and scanning electron microscopic morphology were used to characterize the isolated phages.
Results: Bacteriophage was found in two samples taken from wastewater treatment plants. Phages were proliferated and released under optimal bacterial growth conditions at pH ۷; ۳۷ °C in ۳۶-hour incubation. A ۱۰:۱ ratio of bacterial cells to phage produced the greatest lytic ability. The highest phage titrated under optimal conditions was estimated at ۹×۱۰۹ pfu/ml. The scanning electron microscopic image revealed a bacteriophage morphology corresponding to the head and tail group. The host range observations demonstrated that L. monocytogenes were unique to the isolated phage. The elimination of L. monocytogenes growth in milk and whey samples revealed that this bacteriophage targeted bacteria and resulted in a ۷۵% reduction in bacterial contamination.
Conclusion: The results suggest that the phages obtained from L. monocytogenes may be utilized to combat this pathogen in dairy, agri-food, wastes, and other associated infections.
کلیدواژه ها:
نویسندگان
عبدالرزاق مرزبان
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
مرجان بیرانوند
Department of Biology, Khorramabad Branch. Azad University, Khorramabad, Iran.
سمانه صدیقی خویدک
Medical Biotechnology Research Center, Department of Biology, Ashkezar Branch, Azad University, Ashkezar, Yazd, Iran.
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :