Expression of miR-۴۸۵-۳p and its Target FOXD۳ in Chronic Lymphocytic Leukemia
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 151
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شناسه ملی سند علمی:
JR_JIML-7-1_004
تاریخ نمایه سازی: 6 اسفند 1401
چکیده مقاله:
Background and Aims: Previous investigations have revealed that microRNAs (miRNAs) can function as oncogenes or tumor suppressors in chronic lymphocytic leukemia (CLL) and that the expression of miRNAs, such as miR-۴۸۵-۳p changes in several illnesses. This study was designed to determine the expression level of miR-۴۸۵-۳p and its target FOXD۳ in CLL patients and controls to identify if this miRNA has the potential to be considered as a biomarker in CLL.
Materials and Methods: A total of ۳۵ CLL patients and the same number of controls were recruited. Following total RNA extraction and cDNA synthesis, quantitative analysis was performed by real-time polymerase chain reaction (PCR) using the SYBR Green PCR Master Mix to determine the expression level of miR-۴۸۵-۳p and its target. Moreover, in silico molecular enrichment analysis was conducted on targetome of miR-۴۸۵-۳p.
Results: It was observed that the expression level of miR-۴۸۵-۳p was upregulated in CLL patients compared with controls (p<۰.۰۰۹). However, the expression profile of miR-۴۸۵-۳p’s target (FOXD۳) was significantly downregulated in patients in comparison with the control group (p<۰.۰۲).
Conclusions: Based on the results, miR-۴۸۵-۳p could be a novel biomarker to diagnose CLL and to design novel CLL control strategies by targeting FOXD۳.
کلیدواژه ها:
نویسندگان
فائزه نمازی
Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran.
فاطمه کتابچی
Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
منصور مقیمی
Department of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
نسرین هادی
Medical Genetics Research Center of Genome, Isfahan University of Medical Sciences, Isfahan, Iran.
میرزامحمد رئیسی نیا
Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.
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