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مقالات فارسیISIکنفرانسهاژورنالها

Molecular Detection of Adenoviruses in the Sinus Tissues of Patient by Nested-PCR in Shiraz, Southwest Iran

محل انتشار: مجله بین المللی آزمایشگاه پزشکی، دوره: 6، شماره: 3
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 140

فایل این مقاله در 7 صفحه با فرمت PDF قابل دریافت می باشد

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https://civilica.com/doc/1609584

شناسه ملی سند علمی:

JR_JIML-6-3_005

تاریخ نمایه سازی: 7 اسفند 1401

چکیده مقاله:

Background and Aims: Rhinosinusitis is an inflammation of the mucous membrane that may be caused by infectious agents such as bacteria, fungi and viruses. Few studies have been carried out on the role of viruses in Rhinosinusitis patients .The aim of this study was the molecular detection of Adenoviruses in sinus tissues by nested polymerase chain reaction (PCR) in Shiraz. Materials and Methods: In the present study, ۱۰۳ paraffin-embedded biopsy specimens of sinus tissues were subjected to DNA extraction and tested for adenovirus DNA using Nested PCR. The amplification of a β-globin gene by PCR-based method was used to confirm the quality of extracted DNA. Results: A total of ۱۰۳ samples of sinus tissues were examined. Of these patients, ۵۰ (۴۸.۵۴%) were male and the rest were female (۵۱.۴۶%). The patients’ age ranged between ۲ and ۸۲ years and the mean age was ۴۲.۱۵±۱.۵۶ years. The adenovirus DNA was detected in ۱۳ of ۱۰۳ (۱۲.۶%) samples. Conclusions: The results of this study showed that Adenoviruses have high prevalence in rhinosinusitis patients. As a results, it is an important to investigate clinical significance of viral infections especially Adeno viruses in these patients.

کلیدواژه ها:

Adenovirus ، Nested-PCR ، Rhinosinusitis

نویسندگان

شهاب محمد وند

Department of Bacteriology & Virology, Faculty of Medicine, Shiraz University of Medical Sciences Shiraz, Iran.

محمد کرد

Department of Mycology, Faculty of Medicine, Shiraz University of Medical Sciences Shiraz, Iran.

ندا پیربنیه

Department of Bacteriology & Virology, Faculty of Medicine, Shiraz University of Medical Sciences Shiraz, Iran.

افاق معطری

Department of Bacteriology & Virology, Faculty of Medicine, Shiraz University of Medical Sciences Shiraz, Iran.

سمیه شکری

Department of Virology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

کامران زمردیان

Department of Mycology, Faculty of Medicine, Shiraz University of Medical Sciences Shiraz, Iran.

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