Genotyping of Mycobacterium Tuberculosis Isolated from Suspected Patients in Tehran in ۲۰۱۵-۲۰۱۷

سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 178

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شناسه ملی سند علمی:

JR_JIML-6-3_004

تاریخ نمایه سازی: 7 اسفند 1401

چکیده مقاله:

Background and Aims: Unlike many global efforts to eradicate tuberculosis caused by Mycobacterium, it remains as a life-threatening infection with a worldwide incidence of ۱.۵ million cases each year. However, due to the lack of information about Mycobacterium tuberculosis characterization, more studies are required to evaluate strain diversity and epidemiology of tuberculosis to improve the therapeutic approaches. This study aimed to genotype the Mycobacterium tuberculosis isolated from suspected patients in Tehran, Iran through ۲۰۱۵-۲۰۱۷. Materials and Methods: In the current study, ۳۰ isolates (sputum, broncho-alveolar lavage and biopsy) were collected from different tuberculosis patients at Massoud Clinical Lab of Tehran from ۲۰۱۵ to ۲۰۱۷. To find the single nucleotide polymorphisms and mutated regions, polymerase chain reaction (PCR) was performed on all the isolates to amplify the katG and gyrA genes. Then, PCR products were sequenced and analyzed. Results: The majority of isolates were assigned to PGG۲ (۹۰%), followed by PGG۳ (۱۰%) but no isolate belonging to PGG۱ was found. Conclusions: Our findings demonstrate a remarkable epidemiological pattern of tuberculosis in Tehran. In group ۲, isolates showed a considerably higher frequency compared to isolates in group ۳, which is consistent with other findings reported in Iran. However, in contrast to other Iranian studies, no isolated strains were categorized in principal PGG۱.

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نویسندگان

سعید ذاکر

Department of Biology and Biotechnology, Islamic Azad University, Parand branch, Iran.

هانیه باقریفرد

Department of Microbiology, Islamic Azad University, Damghan branch, Iran.

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