Comparison of the scolicidal effect of Allium sativum and Ferula asafoetida extract on hydatid cyst protoscoleces in vitro
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 158
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شناسه ملی سند علمی:
JR_JZD-6-4_004
تاریخ نمایه سازی: 9 اسفند 1401
چکیده مقاله:
Cystic echinococcosis (CE) is a zoonotic disease and with has a global distribution. Today, much research carries out to inactivate hydatid cyst protoscoleces. In particular, herbal compounds have received more attention due to their cheapness, easy access, low toxicity, and side effects. This study aimed to compare the scolicidal effect of hydroalcoholic extract of Allium sativum (garlic) and Ferula asafoetida (angozeh) on hydatid cyst protoscoleces in vitro. The scolicidal activity of A. sativum and F. asafoetida extracts were evaluated at concentrations of ۵۰, ۱۰۰, ۱۵۰, ۲۰۰, and ۲۵۰ mg/ml following ۱۰, ۳۰, and ۶۰ minutes of exposure. Each reaction was repeated three times. The viability of protoscoleces was examined with a ۰.۱% eosin stain under a light microscope. The chemical composition of two extracts was analyzed using Gas Chromatography-Mass Spectrometry (GC–MS). Statistical analyses were performed with GraphPad software version ۵.۰. The results of this study showed that F. asafoetida extract, at a concentration of ۲۵۰ mg/ml after ۶۰ minutes of exposure, killed ۱۰۰% of the protoscoleces compared to the control group, but the hydroalcoholic extract of A. sativum at the same concentration and time, it was able to kill ۹۸% of protoscoleces. The main chemical components of A. sativum and F. asafoetida identified as allyl methyl trisulfide (۱۲.۸%) and methyl ester (۱۳.۹%), respectively. The findings of the present study showed that F. asafoetida has more potent scolicidal effects than A. sativum. However, further studies are needed to evaluate the effectiveness of the F. asafoetida plant.
کلیدواژه ها:
نویسندگان
Roghayeh Norouzi
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Amin Mohammadpour
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Marzie Hejazy
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Shalaleh Mousavi
Department of Food Hygiene and Aquatic Animals, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
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