Exosomes from adipose-derived stem cells promote angiogenesis and reduce necrotic grade in hindlimb ischemia mouse models
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 26، شماره: 4
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 89
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شناسه ملی سند علمی:
JR_IJBMS-26-4_008
تاریخ نمایه سازی: 9 اسفند 1401
چکیده مقاله:
Objective(s): Acute hindlimb ischemia is a peripheral arterial disease that severely affects the patient’s health. Injection of stem cells-derived exosomes that promote angiogenesis is a promising therapeutic strategy to increase perfusion and repair ischemic tissues. This study aimed to evaluate the efficacy of adipose stem cell-derived exosomes injection (ADSC-Exos) in treating acute mouse hindlimb ischemia.Materials and Methods: ADSC-Exos were collected via ultracentrifugation. Exosome-specific markers were analyzed via flow cytometry. The morphology of exosomes was detected by TEM. A dose of ۱۰۰ ug exosomes/۱۰۰ ul PBS was locally injected into acute mice ischemic hindlimb. The treatment efficacy was evaluated based on the oxygen saturation level, limb function, new blood vessel formation, muscle structure recovery, and limb necrosis grade. Results: ADSC-exosomes expressed high positivity for markers CD۹ (۷۶.۰%), CD۶۳ (۹۱.۲%), and CD۸۱ (۹۹.۶%), and have a cup shape. After being injected into the muscle, in the treatment group, many small and short blood vessels formed around the first ligation and grew down toward the second ligation. The SpO۲ level, reperfusion, and recovery of the limb function are more positively improved in the treatment group. On day ۲۸, the muscle’s histological structure in the treatment group is similar to normal tissue. Approximately ۳۳.۳۳% of the mice had grade I and II lesions and there were no grade III and IV observed in the treatment group. Meanwhile, in the placebo group, ۶۰% had grade I to IV lesions. Conclusion: ADSC-Exos showed the ability to stimulate angiogenesis and significantly reduce the rate of limb necrosis.
کلیدواژه ها:
نویسندگان
Trinh Nguyen
Stem Cell Institute, University of Science Ho Chi Minh City, Viet Nam
Phuc Pham
Stem Cell Institute, University of Science Ho Chi Minh City, Viet Nam
Ngoc Vu
Stem Cell Institute, University of Science Ho Chi Minh City, Viet Nam
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