Isolation and Characterization of Hyperoside-Flavanoidal Glycoside from Rourea Minor for its Anti-Hyperglycemic Activity Potential: An In Vitro Study

To investigate the anti-hyperglycemic activity potential of a hyperoside flavanoidal glycoside isolated from Rourea minor in vitro. The study design entails the preparation of Rourea minor ethanol stem extract, investigation of its extractive values, total flavonoid content, total phenolic content, high-performance thin layer chromatography, gas chromatography, isolation and characterization of flavanoidal glycosides, cytotoxicity testing, a cell-line study, in vitro anti-hyperglycemic activity, and molecular docking analysis using Dipeptidyl-peptidase-IV. The Rourea minor ethanol extract has a high percentage yield (۲۵%). Based on the results obtained from the extractive values, Rourea minor ethanol extract had the highest (۶۲.۶ mg/QA) total flavanoidal content. The phytochemical screening revealed that all of the samples contained phenols, flavonoids, and glycosides confirmed by the thin-layer chromatography results (Rf ۰.۶۶). The gas chromatography identified ۱۵ compounds. The isolated compound from Rourea minor ethanol extract was separated, and the spectra of these fractions with comparable Rf (۰.۶۶) values matched those of the drug, hyperoside (C۲۱H۲۰O۱۲). Hyperoside inhibited the enzymes α-amylase, α-glucosidase, and Dipeptidyl-peptidase-IV in vitro while also acting as an anti-hyperglycemic. As a result, using Auto Dock ۴.۲ software, the presence of hyperoside bound to the Dipeptidyl-peptidase-IV protein was detected at -۶.۹۷ kcal/mol. These findings revealed that Rourea minor has an anti-hyperglycemic effect.