Effects of Ubiquinone on Oxidant and Antioxidant Status in Hepatocellular Carcinoma Cell Line
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 105
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شناسه ملی سند علمی:
JR_JIML-10-1_008
تاریخ نمایه سازی: 16 فروردین 1402
چکیده مقاله:
Background and Aims: The concomitant use of antioxidants during chemotherapy is controversial. It is unknown whether antioxidants increase or decrease the effectiveness of anticancer drugs. Therefore, the present study aimed to investigate ubiquinone's cytotoxic and antioxidant effects on the HepG۲ cell line.
Materials and Methods: The HepG۲ cell line was chosen as an experimental model for hepatocellular carcinoma in this study. The cytotoxic effect of ubiquinone was assessed as a function of time and concentration using the colorimetric MTT assay. The half-maximal inhibitory concentration (IC۵۰) was determined to assess the cytotoxic effects of different ubiquinone concentrations. The protective impacts of ubiquinone on HepG۲ cells were evaluated by assessing the oxidative stress profile.
Results: The MTT showed that the IC۵۰ after treatment with ubiquinone was ۳۵۰ and ۳۳۵ μM at ۲۴ and ۴۸ hours, respectively. Evaluation of redox homeostasis in HepG۲ cells using three doses of ubiquinone, including IC۵۰ and one dose higher and one dose lower than IC۵۰, showed a decrease in oxidative stress and an increase in the antioxidant capacity of HepG۲ cells in a dose-dependent manner (p < ۰.۰۱). An increase in redox hemostasis decreased the viability of HepG۲ cells.
Conclusion: Our results showed ubiquinone could reduce cancer cell survival by interfering in redox oxidative-redox status. Therefore, ubiquinone can be used as an antioxidant supplement along with chemotherapy drugs.
کلیدواژه ها:
نویسندگان
نفیسه حیدری کلوانی
Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
سودابه فلاح
Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
فرشته برجسته
Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
الهام بحرینی
Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
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