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Reproductive status of male rat offspring following exposure to methamphetamine during intrauterine life: An experimental study

محل انتشار: مجله طب تولید مثل ایران، دوره: 21، شماره: 2
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 170

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لینک ثابت به این مقاله:
https://civilica.com/doc/1627474

شناسه ملی سند علمی:

JR_IJRM-21-2_008

تاریخ نمایه سازی: 20 فروردین 1402

چکیده مقاله:

Background: Methamphetamine abuse during pregnancy is associated with maternal and fetal adverse outcomes. Methamphetamine induces reproductive damage in adults; however, its effect has not been studied during pregnancy. Objective: To investigate the effects of methamphetamine exposure during pregnancy on the reproductive system. Materials and Methods: ۱۵ pregnant Wistar rats were divided into ۳ groups (n = ۵/group), and received daily intraperitoneal injections of saline or methamphetamine (۵, and ۱۰ mg/kg) from day ۱۰ until the end of pregnancy. One adult male offspring was selected from each dam. Subjects were euthanized, and their testis was removed. Sperm samples from cauda epididymis were analyzed for sperm concentration, morphology, and motility. Terminal deoxynucleotidyl transferase dUTP nick-end labeling assay was used to detect apoptotic cells. Levels of B-cell lymphoma ۲ protein (Bcl-۲) and Bcl-۲ associated X-protein were measured using Western blot. Results: Methamphetamine significantly decreased sperm concentration (۵ mg vs. saline: p = ۰.۰۰۱, ۱۰ mg vs. saline: p < ۰.۰۰۱), normal sperm morphology (saline vs. ۱۰ mg: p = ۰.۰۰۱), and motility (p: saline vs. ۵ mg = ۰.۰۰۴, ۵ mg vs. ۱۰ mg = ۰.۰۱۱, saline vs. ۱۰ mg < ۰.۰۰۱) in a dose-dependent manner. There was a significantly higher number of terminal deoxynucleotidyl transferase dUTP nick-end labeling -positive cells and higher exposure. Moreover, Bcl-۲ associated X-protein was increased, and Bcl-۲ was decreased in these rats. Conclusion: The present study shows that chronic methamphetamine exposure during intrauterine period can induce apoptosis of seminiferous tubules and decrease sperm quality in adult rats. Moreover, we showed that the intrinsic apoptotic pathway is involved in this process. Further studies are required to identify the complete molecular pathway of these results.

کلیدواژه ها:

Methamphetamine ، Testis ، Fertility ، Reproduction ، Apoptosis ، Intrauterine exposure. ، مت آمفتامین ، بیضه ، باروری ، تولید مثل ، آپوپتوز ، مواجهه درون رحمی.

نویسندگان

Zahra Khoshgoftar Some Saraii

Department of Cellular and Molecular Sciences, Faculty of Advanced Sciences and Technology, Tehran University of Medical Sciences, Islamic Azad University, Tehran, Iran.

Soroush Dianaty

Student Research Committee, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. Universal Scientific Education and Research Network (USERN), Tehran, Iran.

Fatemeh Rouhollah

Department of Cellular and Molecular Sciences, Faculty of Advanced Sciences and Technology, Tehran University of Medical Sciences, Islamic Azad University, Tehran, Iran.

Nayereh Zare

Department of Anatomical Sciences and Cognitive Neuroscience, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.

Batool Ghorbani Yekta

Department of Physiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

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  • Frohmader KS, Bateman KL, Lehman MN, Coolen LM. Effects of ...
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  • Khoradmehr A, Danafar A, Halvaei I, Golzadeh J, Hosseini M, ...
  • Šlamberová R. Review of long-term consequences of maternal methamphetamine exposure. ...
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  • Yamamoto Y, Yamamoto K, Hayase T, Abiru H, Shiota K, ...
  • Alavi SH, Taghavi MM, Moallem SA. Evaluation of effects of ...
  • Nudmamud-Thanoi S, Thanoi S. Methamphetamine induces abnormal sperm morphology, low ...
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  • Lin JF, Lin YH, Liao PC, Lin YC, Tsai TF, ...
  • Sabour M, Khoradmehr A, Kalantar SM, Danafar AH, Omidi M, ...
  • Singh R, Letai A, Sarosiek K. Regulation of apoptosis in ...
  • Galluzzi L, Vitale I, Aaronson SA, Abrams JM, Adam D, ...
  • Sengupta P. The laboratory rat: Relating its age with human’s. ...
  • Campion SN, Carvallo FR, Chapin RE, Nowland WS, Beauchamp D, ...
  • Krzanowska H. The passage of abnormal spermatozoa through the uterotubal ...
  • Sulzer D, Sonders MS, Poulsen NW, Galli A. Mechanisms of ...
  • Ramamoorthy JD, Ramamoorthy S, Leibach FH, Ganapathy V. Human placental ...
  • Barenys M, Gomez-Catalan J, Camps L, Teixido E, de Lapuente ...
  • Fazelipour S, Jahromy MH, Tootian Z, Kiaei SB, Sheibani MT, ...
  • Płotka J, Narkowicz S, Polkowska Ż, Biziuk M, Namieśnik J. ...
  • Wells PG, McCallum GP, Chen CS, Henderson JT, Lee CJJ, ...
  • Joya X, Pujadas M, Falcón M, Civit E, Garcia-Algar O, ...
  • Ganapathy V. Drugs of abuse and human placenta. Life Sciences ...
  • Cordeaux Y, Missfelder-Lobos H, Charnock-Jones DS, Smith GCS. Stimulation of ...
  • Zheng T, Liu L, Shi J, Yu X, Xiao W, ...
  • Slamberová R, Charousová P, Pometlová M. Methamphetamine administration during gestation ...
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