Electrochemical Sensor Based on Molecularly Imprinted Copolymer for Selective and Simultaneous Determination of Ascorbic Acid and Tyrosine

This study reported a electrochemical sensor based on molecularly imprinted polymer (MIP) for simultaneous and selective detection of Ascorbic acid (AA) and Tyrosine (Tyr). The MIP film was electropolymerized on the glassy carbon electrode (GCE) using of o-aminophenol (o-AP) and m-dihydroxy benzene (m-DB) as monomers and the dual analyte of AA and Tyr, and its electrochemical performance was evaluated. Influencing parameters such as the pH value, electropolymerization cycle numbers, and template/monomer ratio were optimized. The differential pulse voltammetry (DPV) technique was used for the simultaneous and individual determination of AA and Tyr in their binary mixture. The introduced sensor showed the linear concentration ranges of ۰.۱-۳۰۰ µM for AA and ۰.۰۱-۱۸۰ µM for Tyr and good limits of detection were ۰.۰۳ µM and ۰.۰۰۳ µM, respectively. The proposed sensor was successfully employed to detect AA and Tyr in real samples. The recoveries were from ۹۷ to ۱۰۵% and the RSD was less than ۳.۵% which exhibited the usability of this sensor in the real sample.