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مقالات فارسیISIکنفرانسهاژورنالها

Evaluating the Genetic Diversity of Helicobacter pylori Isolates in Patients Suffering from Gastritis

محل انتشار: فصلنامه عفونت، اپیدمیولوژی و پزشکی، دوره: 8، شماره: 4
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 153

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لینک ثابت به این مقاله:
https://civilica.com/doc/1635481

شناسه ملی سند علمی:

JR_IEM-8-4_002

تاریخ نمایه سازی: 29 فروردین 1402

چکیده مقاله:

Aim: Helicobacter pylori infections vary in severity and virulence in different populations for various reasons. There are different H. pylori strains with varying degrees of virulence. The genetic diversity of H. pylori strains in gastritis patients in different areas has not been well understood. This study aimed to evaluate the prevalence rate and different genotypes of H. pylori strains in clinical specimens of patients with gastritis in Ilam, Iran. Materials & Methods: Saliva and gastric biopsy samples were collected from ۸۱ patients (۵۵ males and ۲۶ females in the age range of ۲۰ to ۹۰ years) referring to Ilam medical centers. After DNA extraction, the prevalence of H. pylori as well as vacA, cagA, and ureC genes was evaluated using PCR, and then each vacA-positive sample was further evaluated for m۱m۲ and s۱s۲ variants. Findings: The cagA and vacA genes were found in ۲۷ (۷۱%) and ۳۶ (۹۴.۷%) H. pylori-positive samples, respectively. The cagA gene was detected in patients with gastric pain (۴۴.۴%) and anorexia (۱۸.۵۱%). Also, the results showed that the vacA s۲m۲ genotype and m۲ allele were present in ۳۲.۹% of H. pylori isolates. Moreover, s۲m۲ and s۱m۲ genotypes were detected in ۴۲.۱ and ۲۶.۳% of vacA-positive samples, respectively. The lowest frequency was related to the m۱ allele (۱۷.۱۸%). Conclusion: This study results indicate a plausible relationship between the presence of some genotypes of H. pylori and the progression of gastritis, suggesting these markers as promising biomarkers to predict the disease severity.

کلیدواژه ها:

Helicobacter pylori ، Gastritis ، Genotyping ، vacA ، cagA ، PCR

نویسندگان

Sanaz Mami

Department of Immunology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran

Saeedeh Khaleghnezhad

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Masoud Mami

Ilam university of Medical Sciences, Ilam, Iran

masoud dadashi

Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran

Mehdi Goudarzi

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Hossein Ghahremanpour

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Bahareh Hajikhani

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

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