Nano-hesperetin ameliorates ۶-hydroxydopamine-induced behavioral deficits and oxidative damage by up-regulating gene expression of antioxidant enzymes
محل انتشار: مجله گیاهان دارویی ابن سینا، دوره: 13، شماره: 3
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 123
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شناسه ملی سند علمی:
JR_AJP-13-3_007
تاریخ نمایه سازی: 6 اردیبهشت 1402
چکیده مقاله:
Objective: Hesperetin (Hst) has shown several pharmacological effects. The efficacy of Hst is highly restricted in vivo due mainly to poor bioavailability. This investigation was intended to compare the influence of Hst and nano-Hst treatment on ۶-hydroxydopamine (۶-OHDA)-induced behavioral deficits and oxidative stress in rats.Materials and Methods: Forty-two Wistar male rats were equally assigned to ۶ groups: control, ۶-OHDA, Hst۵, Hst۱۰, nano-Hst۵, and nano-Hst۱۰. Treatment with Hst and nano-Hst was initiated ۱ day after the intrastriatal injection of ۶-OHDA and continued for ۲۸ days. Behavioral deficits were evaluated using apomorphine-induced rotation test (AIRT), narrow beam test (NBT) and novel object recognition test (NORT), and the hippocampus and striatum were used to evaluate oxidative stress-related parameters.Results: The rats injected only with ۶-OHDA showed learning and memory deficits but Hst and nano-Hst treatments improved it (p<۰.۰۰۱). Compared to the control group, a marked promotion in Malondialdehyde (MDA) levels along with a marked reduction in activities and gene expression of antioxidant enzymes and reduced glutathione (GSH) levels in the hippocampus and striatum were observed in the ۶-OHDA group (p<۰.۰۱). However, administration of Hst and nano-Hst remarkably diminished MDA levels (p<۰.۰۱), and significantly increased the activities (p<۰.۰۱) and gene expression of antioxidant enzymes (p<۰.۰۵) and GSH levels (p<۰.۰۱) compared to the ۶-OHDA group. In most parameters, nano-Hst has shown better therapeutic effects than Hst.Conclusion: Our findings reveal that Hst can be considered as a potential candidate for the treatment of neurodegenerative diseases and that nano-Hst may have better bioavailability.
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نویسندگان
Akbar Hajizadeh Moghaddam
Department of Animal Sciences, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
Sara Alizadeh
Department of Animal Sciences, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
Monireh Nejadi
Department of Animal Sciences, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
Seyed Reza Mokhtari Sangdehi
Department of Animal Sciences, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
Mahboobeh Zare
Faculty of Herbs, Amol University of Special Modern Technologies, Amol, Iran
Mojtaba Ranjbar
Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran