Evaluation of the Immunogenicity of Recombinant Espb, Espc Proteins from Mycobacterium Tuberculosis and the Fusion Espc/Espb Protein in BALB/C Mice
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 100
فایل این مقاله در 9 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_RBMB-11-4_006
تاریخ نمایه سازی: 6 خرداد 1402
چکیده مقاله:
Background: Two newly identified proteins, EspB and EspC are involved in the pathogenesis of Mycobacterium tuberculosis. The objective of the present study was to evaluate the immunogenicity of recombinant EspC, EspB, and EspC/EspB fusion proteins in mice.
Methods: BALB/c mice were immunized subcutaneously with recombinant EspC, EspB, and fusion EspC/EspB proteins, three times with along with Quil-A as an adjuvant. The cellular and humoral immune responses were evaluated by quantifying IFN-g, IL-۴, IgG, IgG۱, and IgG۲a antibodies against the antigens.
Results: The results showed that the mice immunized with recombinant EspC, EspB, and EspC/EspB proteins did not produce IL-۴, whereas IFN-g was secreted in response to all three proteins. EspC/EspB group produced significant amounts of IFN-g in response to stimulation with all the three recombinant proteins (P<۰.۰۰۱). In mice immunized with EspC, high levels of IFN-g were detected in response to EspC/EspB, and EspC (P<۰.۰۰۰۱); while mice immunized with EspB produced lower levels of IFN- g in response to EspC/EspB, and EspB (P<۰.۰۵).
Conclusions: All the three recombinant proteins induced Th۱-type immune responses in mice against EspB and EspC; however, EspC/EspB protein is more desirable due to the presence of epitopes from both EspC and EspB proteins and the production of immune responses against both.
کلیدواژه ها:
نویسندگان
Omid Salemi
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Zahra Noormohammadi
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Fariborz Bahrami
Department of Immunology, Pasteur Institute of Iran, ۶۹ Pasteur Ave, Tehran ۱۳۱۶۹-۴۳۵۵۱, Iran.
Seyed Davar Siadat
Department of Mycobacteriology and pulmonary research, Pasteur Institute of Iran, ۶۹ Pasteur Ave., Tehran ۱۳۱۶۹-۴۳۵۵۱, Iran.
Soheila Ajdary
Department of Immunology, Pasteur Institute of Iran, ۶۹ Pasteur Ave, Tehran ۱۳۱۶۹-۴۳۵۵۱, Iran.
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :