The effect of Cakile maritima Scop. on kinetic parameters ofcabbage,s guaiacol peroxidase

Peroxidase (PODS; EC ۱.۱۱.۱.X) in different living organisms, including animals, plants, andmicroorganisms have wide applications in various fields of medical biochemistry,biotechnology, food industry and their attractiveness has been increased on the study ofdetoxifying and removal of organic contaminants from wastewaters. The in vitro effect ofmethanol extract of Cakile maritima on the peroxidase was investigated and the kineticparameters were measured. The peroxidase (POD) activity from fresh cabbage extract wasevaluated using the spectrophotometric method. The enzyme activity in the crude extract andfinal dialysate was monitored at ۴۷۰ nm with H۲O۲ as substrate and guaiacol as an electrondonor in phosphate-citrate buffer pH ۵ and at ۵۵oC. For the H۲O۲ substrate, the Km and Vmaxvalues were found as ۰.۳۷ mM and ۶.۰۵ mM/min. The extract of Cakile maritima has shownthe inhibitory effect as noncompetitive on the peroxidase. Therefore, these results could beuseful for the prevention and treatment of certain disease, which peroxidase enzyme plays avital role.