Chitosan Nanoparticles: As an Anti-Biofilm Agent against Acinetobacter Strains baumannii Representing the Multidrug Resistance Phenotype
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 87
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شناسه ملی سند علمی:
JR_SHIMU-31-2_001
تاریخ نمایه سازی: 28 خرداد 1402
چکیده مقاله:
Introduction: Acinetobacter baumannii (A. baumannii) is a non-fermentative gram-negative coccobacillus that shows high resistance to antimicrobial compounds. Biofilm formation is one of the essential features of many Acinetobacter species that leads to high antibiotic resistance. This study aimed to evaluate the ability of biofilm formation and determine the antibiofilm activity of chitosan nanoparticles in clinical isolates of A. baumannii.
Material & Methods: This descriptive cross-sectional study was conducted in ۲۰۲۱ and investigated ۱۰۰ isolates collected from different hospitals. Microscopic, biochemical, and molecular tests were performed to identify the bacteria. The antibiotic resistance pattern of the isolates was evaluated by the disk diffusion method against ۱۰ antibiotics, and the ability to produce biofilm was evaluated by microtiter plate method. Subsequently, ۱۶SrRNA and CsuA genes were identified by multiplex-PCR molecular methods. After the preparation of chitosan nanoparticles and determination of MIC concentration, antibiofilm activity was measured by plate microtiter, and Real-Time PCR was used to examine the expression of the CsuA gene involved in biofilm.
Findings: In this study, out of ۱۰۰ isolates examined, ۲۹ isolates were confirmed as A. baumannii. Among ۲۹ isolates, ceftazidime had the highest drug resistance (۷۵.۸۶%). The CsuA gene was detected in ۵۱.۷۲% of the isolates. Moreover, using a microtiter plate and Real-Time PCR, the level of antibiotic activity of chitosan nanoparticles was determined at a significant level of P<۰.۰۱.
Discussion & Conclusion: Considering the anti-biofilm effects found in the present study, it seems that chitosan nanoparticles can be used as a pharmaceutical candidate in the pharmaceutical industry.
کلیدواژه ها:
نویسندگان
غزل قجری
Dept of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
نگین رجایی
Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
آرجیت چاکرابورتی
National Sports University, Ministry of Youth Affairs and Sports, Government of India, Imphal
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