The Biological role of glycosides in alfalfa (Medicago sativa L.) as a resistance factor against alfalfa weevil (Hypera postica Gyll.)
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 104
فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJGPB-11-1_002
تاریخ نمایه سازی: 28 خرداد 1402
چکیده مقاله:
After the identification of resistant, semi-susceptible, and susceptible genotypes among ۴۲ alfalfa (Medicago sativa L.) genotypes concerning farm traits, nine genotypes were selected. Saponins obtained as a result of homogenous suspension of alfalfa tissue under maximum alfalfa weevil (Hypera postica Gyll.) attack were extracted using methanol and were purified using water-saturated butanol. The resulting solution was immobilized using the active enzyme extracted from the midgut of the pest and the thermally deactivated enzyme. In addition to the isolation of secondary glycosidic metabolites extracted from alfalfa, the enzymatic hydrolysis activity of the pest in the glycosides of genotypes were monitored using thin layer chromatography. The results of the enzymatic digestion test using TLC indicated that other than glycosides۱ epigenin, other compounds were digested in the solution. The flavonoidal band of epigenin glycoside۱ in all five resistant genotypes, including Tak Buteh and Ranger, was high in density and nearly equal. While maintaining the stability of the molecular structure, this compound was isolated and revealed at Rf=۰.۴۵ on a TLC plate. Therefore, midgut enzymes of alfalfa weevil were not able to digest the above compound in alfalfa. In two susceptible genotypes of Poly Cross Shiraz and Mahali Neyshaburi, the saponin band of soysaponin۱ at Rf=۳۷ had the highest density. In natural field conditions and at the time of biological stress, the lowest concentrations of saponin, soysaponin ۱, and the highest concentration of flavonoids, epigenin glycoside ۱, had a significant biological role in resistance to pests, and reduced damage to the cultivars under study.
کلیدواژه ها:
نویسندگان
صلح الدین جمشیدی گولان
Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran.
سید سعید موسوی
Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran.
حمزه مظاهری لقب
Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran.
مجید کزازی
Department of Plant Pathology, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran.
حجت اله مظاهری لقب
Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran.
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :