Antifungal effects of zinc nanoparticles green synthesized by Lavandula angustifolia extract, alone and combined with nystatin against Candida albicans, a major cause of oral candidiasis
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 65
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شناسه ملی سند علمی:
JR_HERM-11-4_011
تاریخ نمایه سازی: 28 تیر 1402
چکیده مقاله:
Introduction: This work aimed to determine the antifungal effects of zinc nanoparticles (ZnNPs) green synthesized by Lavandula angustifolia extract, alone and along with nystatin against Candida albicans.
Methods: ZnNPs were green synthesized with L. angustifolia extract by microwaves method. Antifungal effects of ZnNPs were studied by measuring the minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) using the broth microdilution method based on the modified M۲۷-A۳ protocol on yeasts, recommended by the Clinical and Laboratory Standards Institute (CLSI). Effects of green synthesized ZnNPs against human normal fibroblast-like Gingiva (HGF۱-PI۱) and human epithelial-like oral cancer (KB) cell lines were studied by MTT (۳-(۴,۵-Dimethylthiazol-۲-yl)-۲,۵-diphenyltetrazolium bromide) method.
Results: The ZnNPs showed a spherical shape with some grains of different lengths. The best levels of MIC and MFC were connected to the combination of ZnNPs + nystatin with values of ۰.۲۰۴ and ۰.۲۵۰ μg/mL, respectively. The combination of ZnNPs with nystatin compared to the nystatin group had a significantly better antifungal effect on C. albicans (P < ۰.۰۰۱). The ۵۰% cytotoxic concentrations of ZnNPs against normal (HGF۱-PI۱) and cancer (KB) cells were ۱۷۲.۳ and ۸۳.۲ μg/mL, respectively.
Conclusion: We found that ZnNPs plus nystatin had a potent antifungal effect against C. albicans. These findings indicated the cytotoxic effects of green synthesized ZnNPs on cancerous cells, whereas they were nontoxic for normal cells. Additional studies are necessary to explain the accurate mechanism and toxicity.
کلیدواژه ها:
نویسندگان
Saman Nasiri
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Hossein Mahmoudvand
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Mojtaba Shakibaie
Pharmaceutical Sciences and Cosmetic Products Research Center, Kerman University of Medical Sciences, Kerman, Iran
Seyyed Amir Hossein Mousavi
Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran