Cytotoxicity of Trichoderma Reesei Extract in Hepa۱-۶ Cancer Cells
محل انتشار: مجله علوم پیشرفته زیست پزشکی، دوره: 12، شماره: 1
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 119
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شناسه ملی سند علمی:
JR_JABS-12-1_012
تاریخ نمایه سازی: 31 تیر 1402
چکیده مقاله:
Background & Objective: In the last decades, many successful anticancer fungal metabolites have been obtained, and fungi have shown great potential to produce beneficial anticancer drug compounds. In this article, the effects of the reference strain of Trichoderma reesei ethanolic extract on the Hepa۱-۶ cell line were studied.
Materials & Methods: The fungus was initially cultured in the potato dextrose agar media, and then, it was transferred to yeast peptone dextrose broth. Afterward, the ethanolic and aquatic extracts were prepared, and were analyzed by chromatography and mass spectrometry (GC-MS). Then, the cell lines were treated with different doses of the extracts. The cytotoxic activity of fungal extracts were evaluated by MTT assay, Ferric reducing ability of plasma (FRAP) assay and Ferric Reducing Antioxidant Power (FRAP) Assay. Alkaline phosphatase (ALP) and Lactate dehydrogenase (LDH) measurement tests were done on the culture and cell fractions. Hematoxylin & Eosin staining was performed for microscopic studies, and an oxidant attack-antioxidant defense test was performed on the cell fraction.
Results: GC-MS determined compounds such as cineal, cyclohexene, hydroxyethyl benzaldehyde, terpinenyl acetate, dihydro esthophyllene, propionic acid, hexadecanoic acid, and octadecanoic acid were extracted from the fungal extract. The half maximal inhibitory concentration (IC۵۰) was between ۷-۸%, and the highest cytotoxic effectiveness was ۱۰-۱۵%. An increase in the levels of ALP, LDH enzymes, and total protein was observed.
Conclusion: Therefore, the findings suggest that the extract of T. reesei has inhibitory effects on Hepa۱-۶ cancer cell lines.
کلیدواژه ها:
نویسندگان
سید مهدی سجادی
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
فرزاد کتیرایی
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
امیر علی شهبازی فر
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
حسین طایفی نصرآبادی
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
علی اصغر فخری دمشقیه
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
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