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مقالات فارسیISIکنفرانسهاژورنالها

Evaluation of Phenolic Content, Antioxidant Activity and Cytotoxic Effects of Ulva lactuca and Hypnea musiformis Marine Algae on MDA-MB-۴۶۸ Cell Line

محل انتشار: مجله علوم پیشرفته زیست پزشکی، دوره: 11، شماره: 3
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 90

فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد

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لینک ثابت به این مقاله:
https://civilica.com/doc/1702299

شناسه ملی سند علمی:

JR_JABS-11-3_004

تاریخ نمایه سازی: 31 تیر 1402

چکیده مقاله:

Background & Objective: Breast cancer is one of the leading causes of death among women around the world. Attempts to find alternative therapies continue due to therapeutic resistance, side effects, and the high cost of conventional treatments. Materials & Methods: Hydro-methanolic extract of Ulva lactuca and Hypnea musiformis marine algae was prepared by maceration method. The phenolic content of these extracts was compared using the standard Folin-Ciocateu method and the antioxidant capacity was evaluated by the FRAP method. The cytotoxic effects of algae were compared using MTT assay and morphological changes were evaluated by an inverted microscope. Results: The phenolic content of Hypnea musiformis extract (۱۲.۴۶ ± ۱.۱۹ µg GAE/mg, P<۰.۰۰۰۱), and its antioxidant activity (۲۰۳.۰۳ ± ۲۷.۸۷ µmol Fe۲+/g, P= ۰.۰۰۷), was significantly higher than that of the alga Ulva lactuca. The cytotoxic effects of Hypnea musiformis extract were concentration and time dependent and had severe morphological changes on the cells. Algae extract of Hypnea musiformis (۱۰۰۰ µg/ml) inhibited ۸۴.۷۹ ± ۴.۶۶ of % cell proliferation after ۷۲ hours of treatment. The IC۵۰ value of cytotoxic effect of Hypnea musiformis and Ulva lactuca on MDA-MB-۴۶۸ was respectively ۷۰۱.۲ and >۱۰۰۰ µg/ml after ۷۲h incubation. Conclusions: Hypnea musiformis had higher cytotoxic effects than Ulva lactuca on MDA-MB-۴۶۸ cells, probably due to its higher phenolic content and antioxidant capacity. Therefore, it appears that a Hypnea musiformis alga is a better option to continue research on drug discovery of anticancer compounds.

کلیدواژه ها:

Algae (Ulva lactuca ، Hypnea musiformis) ، Antioxidant ، cytotoxic effects ، Breast cancer

نویسندگان

علیرضا مولازاده

Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran

راضیه رنجبر

Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran

امین دخیلی اردستانی

Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran

عارف کشاورزی

Fars blood transfusion organization research center, Shiraz, Iran

فاطمه کریم زاده

Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran

مریم راهنورد

Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran

زهرا زارعی جلیانی

Department of Marine Biology, University of Hormozgan, Bandar Abbas, Iran

سهراب نجفی پور

Department of Microbiology, Fasa University of Medical Sciences, Fasa, Iran

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