Prevalence of Virulence Factors and Antimicrobial Resistance of Streptococcus agalactiae and Streptococcus uberis in Ruminant Sub-clinical Mastitic Milk in Iran
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 63
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شناسه ملی سند علمی:
JR_JIML-4-1_005
تاریخ نمایه سازی: 14 مرداد 1402
چکیده مقاله:
Background and Aims: The aim of this study was to determine the virulence and antibiotic resistance properties of Streptococcus agalactiae (S. agalactiae) and Streptococcus uberis (S. uberis) isolated from the ruminant’s sub-clinical mastitic milk in Iran.
Materials and Methods: Positive samples in the CMT test were cultured and S. agalactiae and S. uberis have been identified using biochemical tests and polymerase chain reaction (PCR). Virulence and antibiotic resistance genes were studied using PCR. A total of ۳۵۰ milk samples were collected randomly from ۴ major provinces of Iran. We chose ۵۵ bovine, ۴۳ ovine, and ۳۲ caprine herds from these regions.
Results: Totally, ۳۴.۵۲% of bovine, ۲۵% of ovine, and ۲۵% of caprine milk samples were found to be contaminated with S. agalactiae and also, ۲۷.۳۸% of bovine, ۲۵% of ovine, and ۲۵% of caprine milk samples were found to be contaminated with S. uberis. The most commonly detected virulence genes of S. agalactiae were cfb (۶۸.۷۵%), scpB and pavA (۴۳.۷۵%). Also, the most commonly detected pathogenic genes of S. uberis were sua and hasB (۸۳.۳۳%), cfu (۷۵.۰۰%) and skc (۷۰.۸۳%). Totally, tetO (۸۹.۲۸%), ermA (۸۲.۱۴%), mefA (۷۳.۲۱%) and tetM (۶۹.۶۴%) were the most common antibiotic resistance genes in Streptococcus isolates. The most resistance were detected against tetracycline (۸۷.۵%) and erythromycin (۸۳.۹۲%), respectively.
Conclusions: Our results showed that animal milk samples should be controlled, especially in colder seasons of the year in order to study the presences of S. agalactiae and S. uberis.
کلیدواژه ها:
نویسندگان
Hassan Momtaz
Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Mahvash eliyasi
Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Reza Soleimani
Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Ali Jazayeri
Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
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