Reference Interval of Cerebrospinal Fluid-Adenosine Deaminase Enzyme in Apparently Healthy Subjects- A Retrospective Study in India
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 48
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شناسه ملی سند علمی:
JR_JIML-4-1_002
تاریخ نمایه سازی: 14 مرداد 1402
چکیده مقاله:
Background and Aims: The aim of the present study was to re-evaluate the upper limit of normal range (ULN) for adenosine deaminase (ADA) level in cerebrospinal fluid (CSF) of healthy subjects in Indian population.
Materials and Methods: Posteriori strategy was used for reference population selection. Individuals whose CSF samples had come to the laboratory of Bhatia Hospital, Mumbai (India) for ADA estimation during the period of January ۲۰۱۵-October ۲۰۱۶. After database search, ۱۳۰ subjects were selected. Hexokinase method for CSF-glucose, turbidometric method for total protein and enzymatic method using the ADA assay kit BSBE for CSF-ADA was used. Non-parametric methodology recommended by IFCC/CLSI for reference intervals calculation was adopted. The ۲.۵ and ۹۷.۵ percentile formed the lower and upper limits of Reference Interval.
Results: ULN range of CSF-ADA for the total study cohort was ۱۰.۷ U/L, which was quite higher compared to the ULN provided in the reagent kit insert (۵.۰ U/L). Upon gender wise comparison, female showed higher median CSF-ADA activity compared to male (Male-۷.۲; Female-۸.۲ p=۰. ۰۱), but upon application of robust methods, ULN of CSF-ADA did not show significant differences (ULN in Male-۱۲.۳ and in Female-۱۲.۵). Age wise analysis revealed that with increasing age; ULN of CSF-ADA showed increasing till the age of ۵۰ years and then showed decreasing trend with further increase in age.
Conclusions: ADA level of ۱۰.۷ U/L in CSF should be adopted as the upper limit of normal range and gender based partitioning is not recommended. Age should be considered during the interpretation of CSF-ADA results for making this diagnostic test more clinically relevant.
کلیدواژه ها:
نویسندگان
.Shalini N Maksane
Department of Biochemistry, Bhatia Hospital, Mumbai, Maharashtra, India.
Rupali Parikh
Department of Pathology, Bhatia Hospital, Mumbai, Maharashtra, India.
Lakshmi Vaswani
Department of Pathology, Bhatia Hospital, Mumbai, Maharashtra, India.
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