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مقالات فارسیISIکنفرانسهاژورنالها

The Effect of Echinops Lasiolepis Extracts, Native Plant of Yazd Province, on Peripheral Blood Mononuclear Cell Proliferation and IFN-γ Secretion

محل انتشار: مجله بین المللی آزمایشگاه پزشکی، دوره: 1، شماره: 1
سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 56

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https://civilica.com/doc/1713428

شناسه ملی سند علمی:

JR_JIML-1-1_002

تاریخ نمایه سازی: 14 مرداد 1402

چکیده مقاله:

Background and Aims: The present study was performed to investigate the effect of Echinops lasiolepis extract on peripheral blood mononuclear cells (PBMCs) proliferation and interferon (IFN)-&gamma secretion. Materials and Methods: PBMCs were obtained from three healthy volunteers and cultured in presence of Echinops lasiolepis methanol extract in different concentrations of ۰.۱, ۱, ۱۰, ۱۰۰ and ۲۰۰ µg/ml. Lipopolysacharide (LPS) and Phytohaemagglutinin (PHA) were considered as mitogen. BrdU cell proliferation assay was used to determine cell proliferation. The concentration of IFN-&gamma in the PBMCs supernatant was determined using enzyme-linked immunosorbent assay. Results: Most concentrations of Echinops lasiolepis methanol extract showed inhibitory effect on PBMCs proliferation. There were significant differences between the concentrations of ۲۰۰, ۱ and ۰.۱ µg/ml (p<۰.۰۵). The optimum proliferation (۳۰.۶۶±۲۴.۶۷%) was obtained at concentration ۱ µg/ml. The PBMCs supernatant IFN-&gamma concentrations in extract treated group without PHA and LPS and in PHA activated group were not significantly different. Conclusions: Our findings indicated that Echinops lasiolepis extract has immunomodulating activity which may be potentialy to used as an immunotherapy agent.

کلیدواژه ها:

Cell proliferation ، Echinops lasiolepis ، IFN-γ ، Phytohaemagglutinin ، تکثیر سلولی ، شکر تیغال اردستانی ، اینترفرون گاما

نویسندگان

مریم اسدی

Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

حسین هادی ندوشن

Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

سید علی میرغنی زاده

Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

علیرضا کریم الله

Department of Pharmacology, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

فاطمه سادات دشتی

Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

سعید ملک حسینی

Department of Immunology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

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