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مقالات فارسیISIکنفرانسهاژورنالها

The effect of ox-LDL and platelets on macrophages, M۲ macrophage polarization, and foam cell formation

محل انتشار: مجله آریا آترواسکلروز، دوره: 19، شماره: 1
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 98

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https://civilica.com/doc/1714927

شناسه ملی سند علمی:

JR_RYA-19-1_004

تاریخ نمایه سازی: 15 مرداد 1402

چکیده مقاله:

Background: The accumulation of oxidized LDL (ox-LDL) in macrophages in association with platelet activity leads to the formation of foam cells, which play a key role in the pathophysiology of atherosclerosis and coronary artery diseases (CAD). Here, in this study, we aimed to investigate the simultaneous effect of ox-LDL and platelets on foam cell formation, as well as modification in cell markers. Methods: First, the U۹۳۷, a human monocytic cell line,  was cultured in RPMI-۱۶۴۰. Then, isolated platelets were co-cultured with the U۹۳۷ and exposed to ox-LDL (۸۰ µg/ml) to evaluate the impact of ox-LDL on foam cell formation using Oil red O (ORO) staining. Also, the expression of foam cells’ surface markers and CD۳۶, ABCA۱, SR-B۱, ACAT۱, and LXRα genes, which are involved in macrophage metabolism and ox-LDL uptake, was measured by flow cytometry and real-time PCR, respectively. Results: Our findings suggest that platelets promoted foam cell formation (ORO-positive cells), accompanied by a higher level of CD۱۶۳+ M۲ macrophages. Furthermore, the expression of CD۳۶, ABCA۱, SR-B۱, ACAT۱, and LXRα genes, which are implicated in cholesterol accumulation in macrophages, was significantly upregulated in the ox-LDL+ platelets group compared to the control (P < ۰.۰۵). Moreover, the up-regulation of CD۳۶, ABCA۱, and SR-B۱ genes in the ox-LDL+ platelets group was more accentuated compared to the ox-LDL group (P < ۰.۰۵).Conclusions: Owing to the positive effector role of platelets in the formation of foam cells and CD۱۶۳+ cells, it could be assumed that platelets play a dual role in the development of these cells.

کلیدواژه ها:

ox-LDL ، Platelets ، Macrophages ، Foam cells ، Atherosclerosis

نویسندگان

Fatemeh Barati

Department of Hematology and Blood Banking, School of Allied Medical Sciences Shahid Beheshti University of Medical Sciences, Tehran, Iran

Davood Bashash

Department of Hematology and Blood Banking, School of Allied Medical Sciences Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mohamad Hosein Mohamadi

HSC Research Center-Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical sciences, Tehran, Iran

Mahdieh Mehrpori

Department of Laboratory Sciences, School of Allied Medical Sciences Alborz University of Medical Sciences, Karaj, Iran

Mohsen Hamidpour

HSC Research Center-Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical sciences, Tehran, Iran

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