Investigation of Effective Factors on the Optimization of Surface Layer Protein Production in the Deinococcus radiodurans R۱ Strain using Response Surface Method
سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 59
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شناسه ملی سند علمی:
JR_IJMM-11-3_007
تاریخ نمایه سازی: 16 مرداد 1402
چکیده مقاله:
Background and Aims: Due to its self-assembly properties on variety of surfaces and creating regular functional groups, surface layer protein isolated from bacteria, has significant applications in the field of nanobiotechnology such as biosensor production, targeting drug delivery systems and tissue engineering. In this research, optimization of discontinuous culture medium compositions for the production of HPI surface layer protein from Deinococcus radiodurans R۱ strain was performed using the response surface (RSM) method.
Materials and Methods: In ۲۰۱۶, culture medium for ۱۶ designed experiments with fractional factorial analysis (FFA) was prepared and effective factors among six variables of the discontinuous culture medium was investigated for the production of surface layer proteins of D.radiodurans R۱. Twenty experiments were then designed for the optimization of effective variables using central composite design (CCD) method. Surface protein purity was assessed using SDS-PAGE analysis and its concentration was calculated via Bradford method.
Results: The optimized medium containing ۱۳.۳۶ g/L glucose, ۵ g/L yeast extraction, ۵ g/L tryptone, ۲ g/L HEPES buffer, ۰.۵۵ MgSO۴*۷H۲O, ۰.۰۳۶۸ g/L MnCl۲*۴ H۲O, was determined. Wet cellular mass was found as ۱۶.۸۷ g/L, which is ۲۴% more than TGY basic medium and ۷۴% much more than TYG culture medium including NaCl.
Conclusions: Results of the Bradford method demonstrated that the concentration of surface layer protein HPI isolated from D. radiodurans R۱ was ۵.۶ mg which was more than twice of that using ۱liter of basic TGY medium.
کلیدواژه ها:
Deinococcus radiodurans R۱ ، S-layer protein ، Response surface methodology (RSM) ، دینوکوکوس رادیودورانس R۱ ، پروتئین لایه سطحی ، روش سطح پاسخ (RSM)
نویسندگان
Behruz Ebadi Sharaf abad
Department of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran
Rassoul Khalilzadeh
Department of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran
Mahdi Alijanianzadeh
Department of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran
Maryam Abdolirad
Department of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran
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