Study of Lipase genes expression (Lip۲,۳,۵) in Candida albicans isolated from patients

سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 65

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شناسه ملی سند علمی:

JR_IJMM-11-2_007

تاریخ نمایه سازی: 16 مرداد 1402

چکیده مقاله:

Background and Aims: Candida, is a fungal flora in human that can be colonized on the skin and mucosal surfaces. Various factors are involved in causing disease by candida, one of the most important of these factors, are secreted hydrolytic enzymes and aspartyl proteinase enzymes are as a potential factor, but other secretion of hydrolytic enzymes such as phospholipase and lipase are also important pathogen factors. In this study, gene expression of LIP۲, LIP۳ and LIP۵ were studied in isolated C. albicans in ۱۰۰ samples from patients. Materials and Methods: A total of ۱۰۰ strains of C. albicans was isolated in ۲۰۱۵-۲۰۱۶ years by morphology and molecular methods, from clinical samples. After extracting RNA from them by RNX-Plus kit, expression of LIP۲, LIP۳ and LIP۵genes was evaluated by using RT-PCR test. Results: From ۱۰۰ clinical isolates of C. albicans, LIP۲ gene expressed ۵۵%, LIP۳ gene ۴۱% and LIP۵ gene ۴۸%. Among the positive samples, ۲۴ cases (۳۳.۸۰%) were expressed three genes at the same time. Conclusions: In this study, frequency of lipase genes was different in strains of C.albicans. Lipase gene was not expressed in ۳۰% of cases. It can be concluded that other genes than lipase gene can be involved in the pathogenesis.

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نویسندگان

Mansoureh Ghaffari

Department of Microbiology, Biological Science College, Varamin-pishva branch, Islamic Azad University,Varamin-Pishva, Iran

Fatemeh Noorbakhsh

Department of Microbiology, Biological Science College, Varamin-pishva branch, Islamic Azad University,Varamin-Pishva, Iran

Reza Kachuei

Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

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