Prevalence of norA and norB efflux pump genes in clinical isolates of Staphylococcus aureus and their contribution in ciprofloxacin resistance
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 65
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شناسه ملی سند علمی:
JR_IJMM-10-5_003
تاریخ نمایه سازی: 16 مرداد 1402
چکیده مقاله:
Background and Aim: Recently, ciprofloxacin resistance in Staphylococcus aureus strains due to efflux pumps has become a significant challenge. This study was performed to evaluate the frequency of norA and norB efflux pump genes and their roles in resistance to ciprofloxacin in clinical isolates of S. aureus.
Materials and Methods: A total of ۲۵۰ clinical samples were collected from different hospitals Tehran-Iran during ۲۰۱۵-۲۰۱۶ and S. aureus isolates were identified by standard microbiological tests. Antimicrobial susceptibility patterns were determined by disk diffusion method using CLSI guideline. Subsequently, the presence of norA and norB efflux pump genes in ciprofloxacin isolates were detected using PCR method. Finally, active efflux pumps were evaluated using ciprofloxacin and ethidium bromide MICs.
Results: Among ۲۵۰ clinical samples, ۵۰ S. aureus isolates were recovered and the results of antibiotic susceptibility tests showed the ۳۴ out of ۵۰ S. aureus isolates (۶۸%) were resistant to methicillin (MRSA) and from the ۳۴ MRSA, ۱۲ isolates (۲۴%) were resistant to ciprofloxacin. Moreover, the norA and norB genes were found in ۱۰۰% and ۸۳% of ciprofloxacin resistant isolates, respectively.
Conclusions: The results of this study show the potential role played by norA and norB efflux pumps in the development of resistance to ciprofloxacin in clinical isolates of S. aureus and the detection of these genes could be important for the suggestion of an effective treatment model for the S. aureus infections.
کلیدواژه ها:
Staphylococcus aureus ، Efflux pump ، nor A ، norB ، استافیلوکوکوس اورئوس ، پمپ افلوکس ، norA ، norB
نویسندگان
Mohadeseh Alsadat Haddadi Zahmatkesh
Department of Biology, Tehran North Branch, Islamic Azad University, Tehran, Iran
Mohadeseh Laripoor
Department of Biology, Tehran North Branch, Islamic Azad University, Tehran, Iran
Amir Mirzaie
Young Researchers Club and the elite, East Tehran Branch, Islamic Azad University, Tehran, Iran
Fatemeh Ashrafi
Department of Biology, Tehran North Branch, Islamic Azad University, Tehran, Iran
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