Relationship between presence of genes encoding ESBLs and antimicrobial susceptibility pattern in Escherichia coli clinical isolates
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 60
فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJMM-10-1_002
تاریخ نمایه سازی: 16 مرداد 1402
چکیده مقاله:
Background and aims: Escherichia coli harboring extended spectrum β-lactamase (ESBLs) are significantly resistant to other antibiotics and caused several health problems. The present study was carried out to characterize the ESBL types and to determine their pattern of resistance to four antimicrobial agents in clinical isolates of E. coli.
Material and methods: During April ۲۰۱۲ to May ۲۰۱۳, ۵۰۰ clinical isolates of E. coli were collected from two hospitals in Boroujerd. Phenotypic screening and confirmation tests for ESBL detection and antibiotic susceptibility testing were performed according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Minimum inhibitory concentration of imipenem, meropenem, amikacin and ceftriaxone was determined by the E-test method. All ESBLs producing isolates were examined for presence of the TEM, SHV and CTX-M genes by PCR method.
Results: All of the studied isolates were susceptible to imipenem and meropenem. Additionally, among ESBLs producer isolates good susceptibility to amikacin and ceftriaxone (P-value ˂ ۰.۵) was observed. The prevalence of the TEM, SHV and CTX-M genes among ۱۹۰ ESBLs producing isolates was as follows: TEM positive: ۶۱ (۳۲.۱%), SHV positive: ۶۰ (۳۱.۶%) and CTX-M: ۶۲ (۳۲.۶%). TEM, SHV and CTX-M genes occurred together in ۲۵ (۱۳.۱%) of the isolates.
Conclusion: The high prevalence of E.coli producing ESBL in Boroujed hospitals reported. More than one ESBL was produced by many isolates, and this was correlated with increased resistance levels. Carbapenems continue to show good in vitro activity agent ESBLs producing organism.
کلیدواژه ها:
نویسندگان
Mohsen Mirzaee
Department of Laboratory Sciences, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran
Roohangiz Eftekhari
Microbiology ward, Emmam khomeni Hospital. Boroujerd, Iran.
Neda Taghizadeh
Department of Microbiology, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran.
Mohammad Reza Mehrabi
Department of Laboratory Sciences, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :