ورود
مقالات فارسیISIکنفرانسهاژورنالها

Effect of Melatonin on Alpha Synuclein and Autophagy in Dopaminergic Neuronal Differentiation of Adipose Mesenchymal Stem Cells

محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 12، شماره: 1
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 73

فایل این مقاله در 14 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1736001

شناسه ملی سند علمی:

JR_RBMB-12-1_002

تاریخ نمایه سازی: 31 مرداد 1402

چکیده مقاله:

Background: The current work investigated the effect of melatonin on differentiation of adipose mesenchymal stem cells (AD-MSCs) into dopamine producing cells and its effect on autophagy process and alpha-Synuclein (α-Syn) secretion.  Methods: AD-MSCs were characterized by flow cytometry and divided into ۴ groups; i) control group (AD-MSCs without any treatment), ii) M+MSCs group (MSCs treated with ۱ µM melatonin for ۱۲ days), iii) DN group (MSCs cultured in neurobasal A medium and essential neuronal growth factors for ۱۲ days) and iv) DN+M group (MSCs cultured in neurobasal A medium and ۱µM melatonin for ۱۲ days. By the end of experiments, the dopamine and α-Syn levels using ELISA, the expression of MAP-۲, m-TOR and α-Syn genes at the level of mRNA and detection of autophagosomes formation using transmission electron microscope were performed.  Results: We found that the isolated cells were MSCs due to their positivity expression for CD۱۰۵ and CD۹۰ and negativity expression for CD۳۴ and CD۴۵. The concentration of dopamine was significantly higher and α-Syn concentration was significantly lower in DN+M group when compared to other groups (P< ۰.۰۰۵). Also, this group showed the highly expression for MAP-۲ gene and less expression for m-TOR and α-Syn genes (P< ۰.۰۰۵). Moreover, there was significantly increase in autophagosomes formation in this group than another group (P< ۰.۰۰۵).  Conclusions: It is concluded that the melatonin promotes the differentiation of rat AD-MSCs into dopaminergic cells via induction of autophagy process and reduction of α-Syn secretion.

کلیدواژه ها:

Alpha synuclein ، Autophagy ، Dopaminergic cells ، Melatonin ، Mesenchymal stem cells.

نویسندگان

Hadeer Ahmed Mohamed Ibrahim

Urology and Nephrology Center, Mansoura University, Mansoura, Egypt.

Abdelaziz Mohamed Hussein

Department of Medical Physiology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.

Mahmoud Gabr

Urology and Nephrology Center, Mansoura University, Mansoura, Egypt.

Rasha Aly El-Saeed

Division of Biochemistry, Chemistry Department, Faculty of Sciences, Mansoura University, Mansoura, Egypt.

Omar Abd-Alhakem Ammar

Basic Science Department, Delta University for Sciences and Technology, Gamasa, Egypt.

Ahmed Abdulatif Hassan Mosa

Department of Neurology, Faculty of Medicine, Delta University for Sciences and Technology, Gamasa, Egypt.

Abdel-Aziz Fatouh Abdel-Aziz

Division of Biochemistry, Chemistry Department, Faculty of Sciences, Mansoura University, Mansoura, Egypt.

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • Askar MH, Hussein AM, Al-Basiony SF, Meseha RK, Metias EF, ...
  • Alizadeh R, Bagher Z, Kamrava SK, Falah M, Hamidabadi HG, ...
  • Alizadeh R, Hassanzadeh G, Joghataei MT, Soleimani M, Moradi F, ...
  • Hussein AM, Eldosoky M, El-Shafey M, El-Mesery M, Ali AN, ...
  • Scrivo A, Bourdenx M, Pampliega O, Cuervo AM. Selective autophagy ...
  • Chen HX, Liang F, Gu P, Xu PL, Xu HJ, ...
  • Mostafavi H, Forouzandeh M, Bigdeli MR, Nadri S, Eskandari M. ...
  • Chung TH, Hsu SC, Wu SH, Hsiao JK, Lin CP, ...
  • Alajez NM, Al-Ali D, Vishnubalaji R, Manikandan M, Alfayez M, ...
  • Muñoz MF, Argüelles S, Medina R, Cano M, Ayala A. ...
  • Hasani S, Boroujeni ME, Aliaghaei A, Baghai K, Rostami A. ...
  • Lerner AB, Case JD, Mori W, Wright MR. Melatonin in ...
  • Govindasamy N, Chung Chok K, Ying Ng P, Yian Koh ...
  • Mortezaee K, Khanlarkhani N, Sabbaghziarani F, Nekoonam S, Majidpoor J, ...
  • Zhao L, Hu C, Zhang P, Jiang H, Chen J. ...
  • Mohammadi-Mahdiabadi-Hasani MH, Nabiuni M, Parivar K, Yari S, Sahebi AR, ...
  • Phonchai R, Phermthai T, Kitiyanant N, Suwanjang W, Kotchabhakdi N, ...
  • Morris JK. A formaldehyde glutaraldehyde fixative of high osmolality for ...
  • Chia SJ, Tan EK, Chao YX. Historical Perspective: Models of ...
  • Zomer HD, Roballo KC, Lessa TB, Bressan FF, Gonçalves NN, ...
  • Zhan XS, El-Ashram S, Luo DZ, Luo HN, Wang BY, ...
  • Bourebaba L, Michalak I, Baouche M, Kucharczyk K, Marycz K. ...
  • Elashry MI, Gegnaw ST, Klymiuk MC, Wenisch S, Arnhold S. ...
  • Machado AK, Homrich SG, Rodrigues CCR, Azzolin VF, Duarte MMMF, ...
  • Bajek A, Gurtowska N, Olkowska J, Kazmierski L, Maj M, ...
  • Mo M, Wang S, Zhou Y, Li H, Wu Y. ...
  • Hernández R, Jiménez-Luna C, Perales-Adán J, Perazzoli G, Melguizo C, ...
  • Moazen B, Zarrinhaghighi A, Nejatollahi F. Selection and Evaluation of ...
  • Khademizadeh M, Messripour M, Ghasemi N, Momen Beik F, Movahedian ...
  • Urrutia DN, Caviedes P, Mardones R, Minguell JJ, Vega-Letter AM, ...
  • Thangnipon W, Puangmalai N, Suwanna N, Soi-Ampornkul R, Phonchai R, ...
  • Liu J, Zhou H, Fan W, Dong W, Fu S, ...
  • López-Armas G, Flores-Soto ME, Chaparro-Huerta V, Jave-Suarez LF, Soto-Rodríguez S, ...
  • Shu T, Wu T, Pang M, Liu C, Wang X, ...
  • Yousefi B, Sanooghi D, Faghihi F, Joghataei MT, Latifi N. ...
  • Riffelmacher T, Simon AK. Mechanistic roles of autophagy in hematopoietic ...
  • Tang AH, Rando TA. Induction of autophagy supports the bioenergetic ...
  • Wan Y, Zhuo N, Li Y, Zhao W, Jiang D. ...
  • Sotthibundhu A, Nopparat C, Natphopsuk S, Phuthong S, Noisa P, ...
  • Liu C, Zhou W, Li Z, Ren J, Li X, ...
    • نمایش کامل مراجع