Inhibitory Effect of Supernatant and Lysate of Saccharomyces cerevisiae on Expression of exoA Gene of Pseudomonas aeruginosa

سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 82

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شناسه ملی سند علمی:

JR_IJMM-17-3_002

تاریخ نمایه سازی: 1 شهریور 1402

چکیده مقاله:

Background and Aim: Pseudomonas aeruginosa is an important ubiquitous and especially common pathogen in the hospital. Exotoxin A that encoded by exoA gene has a role in pathogenesis of this bacterium. Today, probiotics are widely used in the treatment and prevention of diseases. The present study aimed to study the Saccharomyces cerevisiae S۳ effect on the expression of exoA gene. Materials and Methods: S. cerevisiae S۳ supernatant and lysate were prepared. Subminimum inhibitory concentrations (sub-MIC) of extracts were used to P. aeruginosa PAO۱. The level of exoA expression was measured with real-time PCR method. Results: Lysate extract had a reducing effect on toxin gene expression, but unlike lysate, supernatant had an increasing effect on gene expression. Conclusion: We demonstrated that S. cerevisiae S۳ had an inhibitory effect on Exotoxin A virulence factor of P. aeruginosa. We suggest doing more experiments on the effect of S. cerevisiae on other virulence factors of P. aeruginosa and pathogens.

نویسندگان

Zahra Ghorbani

Department of Microbiology, School of Medicine, Shahed University, Tehran, Iran

Maryam Koupaei

Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran

Horieh Saderi

Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran

Seyed Mahmoud Amin Marashi

Department of Microbiology, Qazvin University of Medical Sciences, Qazvin, Iran

Zahra DehghanZadeh

Department of Microbiology, School of Medicine, Shahed University, Tehran, Iran

Parviz Owlia

Molecular Microbiology Research Center (MMRC), Shahed University, Tehran, Iran

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